Detection of Pseudomonas aeruginosa in 5,021 hospitalized pediatric patients presenting with acute respiratory tract infections utilizing targeted next-generation sequencing.

IF 2.9 3区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Clinica Chimica Acta Pub Date : 2026-01-01 Epub Date: 2025-08-06 DOI:10.1016/j.cca.2025.120529

Chunyun Fu, Junming Lu, Xiangjun Lu, Yanhua Feng, Huan Zhang, Wenting Tang, Qiang Huang, Ya Huang, Lishai Mo, Huiping Huang, Qifei Li, Jie Tan

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引用次数: 0

Abstract

Objective: This study aimed to assess the infection status and clinical manifestations of Pseudomonas aeruginosa (P.a.) in hospitalized children with respiratory tract infections.

Methods: Between April 2021 and November 2023, a total of 5,021 hospitalized children with acute respiratory tract infections were collected at the Guangxi Maternal and Child Health Hospital. tNGS was used to detect pathogens in their respiratory samples.

Results: Among the 5,021 hospitalized children with acute respiratory tract infections, P.a. was detected in 113 cases, with a detection rate of 2.25 %. The infected children were mainly infants under 1 year old, and males were predominant. Among the 113 P.a. positive cases, there was 1 case of single P.a. infection and 112 cases of co-infection with other pathogens. In infection patterns, the P.a.-bacteria-virus co-infection is relatively common. A total of 53 different pathogens were identified in children with mixed P. aeruginosa infections, with Human Herpesvirus 5 (observed in 40 cases) being the most prevalent, followed by Acinetobacter baumannii (39 cases) and Mycoplasma pneumoniae (24 cases). Among children with P. aeruginosa infection, the most common respiratory complications included sinusitis, respiratory failure, and pulmonary consolidation. In contrast, the most frequent complications affecting other systems were electrolyte disturbances, gastrointestinal dysfunction, anemia, and myocardial damage. The median duration of hospitalization for the 113 children was 8 days. Out of 113 children with P.a. infection, 55 cases (48.67 %) required respiratory support and 18 cases (15.93 %) required intensive care unit (ICU) admission.

Conclusion: The detection rate of P.a. in hospitalized children with respiratory tract infections was 2.25 %. Almost all hospitalized children with P.a. respiratory tract infections had co-infections with other pathogens. The median duration of hospitalization was 8 days. 15.93 % of hospitalized children with P.a. infection required intensive care.

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利用靶向新一代测序技术检测5021例急性呼吸道感染儿科住院患者的铜绿假单胞菌

目的：了解住院呼吸道感染患儿铜绿假单胞菌（p.a.）的感染状况及临床表现。方法：收集2021年4月至2023年11月广西妇幼保健院急性呼吸道感染住院患儿5021例。使用tNGS检测呼吸道样本中的病原体。结果：5021例急性呼吸道感染住院患儿中检出P.a. 113例，检出率为2.25 %。感染儿童以1 岁以下婴幼儿为主，以男性为主。在113P.a。阳性病例中，单一P.a.感染1例，合并其他病原体感染112例。在感染模式中，单胞杆菌-细菌-病毒合并感染相对常见。在铜绿假单胞菌混合性感染患儿中共鉴定出53种不同的病原菌，其中以人疱疹病毒5型（40例）最为常见，其次是鲍曼不动杆菌（39例）和肺炎支原体（24例）。在感染铜绿假单胞菌的儿童中，最常见的呼吸道并发症包括鼻窦炎、呼吸衰竭和肺实变。相反，影响其他系统的最常见并发症是电解质紊乱、胃肠功能障碍、贫血和心肌损伤。113名儿童的住院时间中位数为8 天。113例P.a.感染患儿中，55例（48.67 %）需要呼吸支持，18例（15.93 %）需要入住重症监护病房（ICU）。结论：呼吸道感染住院患儿P.a.检出率为2.25% %。几乎所有因P.a.呼吸道感染住院的儿童都与其他病原体合并感染。中位住院时间为8 天。15.93 住院儿童P.a.感染需要重症监护。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinica Chimica Acta 医学-医学实验技术

CiteScore

10.10

自引率

2.00%

发文量

1268

审稿时长

23 days

期刊介绍： The Official Journal of the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) Clinica Chimica Acta is a high-quality journal which publishes original Research Communications in the field of clinical chemistry and laboratory medicine, defined as the diagnostic application of chemistry, biochemistry, immunochemistry, biochemical aspects of hematology, toxicology, and molecular biology to the study of human disease in body fluids and cells. The objective of the journal is to publish novel information leading to a better understanding of biological mechanisms of human diseases, their prevention, diagnosis, and patient management. Reports of an applied clinical character are also welcome. Papers concerned with normal metabolic processes or with constituents of normal cells or body fluids, such as reports of experimental or clinical studies in animals, are only considered when they are clearly and directly relevant to human disease. Evaluation of commercial products have a low priority for publication, unless they are novel or represent a technological breakthrough. Studies dealing with effects of drugs and natural products and studies dealing with the redox status in various diseases are not within the journal''s scope. Development and evaluation of novel analytical methodologies where applicable to diagnostic clinical chemistry and laboratory medicine, including point-of-care testing, and topics on laboratory management and informatics will also be considered. Studies focused on emerging diagnostic technologies and (big) data analysis procedures including digitalization, mobile Health, and artificial Intelligence applied to Laboratory Medicine are also of interest.