Effects of manual acupuncture on fatty acid metabolism in a rat model of diarrhea-predominant irritable bowel syndrome: a multi-tissue metabolomic and plasma lipidomic investigation.

IF 2.6 3区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE
Acupuncture in Medicine Pub Date : 2025-08-01 Epub Date: 2025-08-08 DOI:10.1177/09645284251365650
Ying Zhou, Lin Zhang, Tingting Xia, Xuemei Zhang, Lele Zhang, Lifeng Han, Xiumei Gao
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引用次数: 0

Abstract

Objective: Diarrhea-predominant irritable bowel syndrome (IBS-D) is a subtype of IBS. Many patients are dissatisfied with the efficacy of drug treatment and seek complementary and alternative medical therapies, especially acupuncture treatment. In this study, we evaluated the effect of manual acupuncture (MA) on IBS-D using a rat model and investigated the underlying mechanism of action by analyzing the changes in untargeted metabolomics of plasma/liver/colon and targeted plasma lipidomics.

Methods: Thirty male Sprague-Dawley rats were divided into Control, IBS-D and MA groups (n = 10 each). IBS-D/MA groups received acetic acid enemas combined with restraint stress. The Control group received saline enemas. The MA group received daily MA at ST36 for 2 weeks after modeling. Pain and stool quality were measured by the abdominal withdrawal reflex (AWR) and Bristol stool form scale (BSFS). Plasma/liver/colon samples were collected for ultra-high performance liquid chromatography (UHPLC)/Q-Orbitrap mass spectromy (MS) untargeted metabolomics. Plasma was also subjected to UHPLC-MS/MS targeted lipidomics.

Results: MA treatment improved BSFS and AWR scores in IBS-D model rats, suggesting reduced intestinal hypersensitivity. Metabolomic results showed that MA impacted 18 metabolites in the plasma, liver and colon, which were mainly involved in arachidonic acid metabolism, biosynthesis of unsaturated fatty acids, and amino sugar and nucleotide sugar metabolism. Targeted lipidomic results showed MA restored the low levels of four medium-chain and one long-chain fatty acids to normal levels in IBS-D, suggesting that acupuncture treatment of IBS-D involves fatty acid metabolic pathways.

Conclusion: MA reduced markers of intestinal hypersensitivity and impacted a number of metabolic pathways in a rat model of IBS-D.

手针刺对腹泻型肠易激综合征大鼠模型脂肪酸代谢的影响:多组织代谢组学和血浆脂质组学研究。
目的:腹泻型肠易激综合征(IBS- d)是IBS的一种亚型。许多患者对药物治疗的效果不满意,寻求补充和替代医学治疗,特别是针灸治疗。本研究采用大鼠模型,通过分析血浆/肝脏/结肠非靶向代谢组学和靶向血浆脂质组学的变化,评估了手工针刺(MA)对IBS-D的影响,并探讨了其作用机制。方法:雄性sd大鼠30只,随机分为对照组、IBS-D组和MA组,每组10只。IBS-D/MA组采用醋酸灌肠联合约束应激。对照组给予生理盐水灌肠。造模后2周,MA组每天在ST36接受MA治疗。采用腹部戒断反射(AWR)和布里斯托大便形态量表(BSFS)测量疼痛和大便质量。收集血浆/肝脏/结肠样品进行超高效液相色谱(UHPLC)/Q-Orbitrap质谱(MS)非靶向代谢组学分析。血浆也进行UHPLC-MS/MS靶向脂质组学。结果:MA治疗可改善IBS-D模型大鼠的BSFS和AWR评分,提示肠道超敏反应减轻。代谢组学结果显示,MA影响了血浆、肝脏和结肠中的18种代谢物,主要参与花生四烯酸代谢、不饱和脂肪酸的生物合成以及氨基糖和核苷酸糖的代谢。靶向脂质组学结果显示,MA可使IBS-D患者4种低水平的中链脂肪酸和1种长链脂肪酸恢复到正常水平,提示针灸治疗IBS-D涉及脂肪酸代谢途径。结论:MA降低了IBS-D大鼠模型的肠道超敏标志物,并影响了多种代谢途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Acupuncture in Medicine
Acupuncture in Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
4.70
自引率
4.00%
发文量
59
审稿时长
6-12 weeks
期刊介绍: Acupuncture in Medicine aims to promote the scientific understanding of acupuncture and related treatments by publishing scientific investigations of their effectiveness and modes of action as well as articles on their use in health services and clinical practice. Acupuncture in Medicine uses the Western understanding of neurophysiology and anatomy to interpret the effects of acupuncture.
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