Synthesis of zinc-selenium nanocomposites and its hormetic effect on physio-morphological parameters, antioxidant and fatty acid composition of Brassica napus L.

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

3 Biotech Pub Date : 2025-09-01 Epub Date: 2025-08-05 DOI:10.1007/s13205-025-04428-w

Zohaib Younas, Laiba Fatima, Ilyas Ahmad, Zia-Ur-Rehman Mashwani

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引用次数: 0

Abstract

The present study investigated the hormetic effect of Cobalt-60 (⁶⁰Co) gamma irradiation treatment and zinc-selenium nanocomposites on two canola (Brassica napus L.) varieties. Zinc-Selenium nanocomposites (Zn-Se NCs) was synthesized from Allium sativum and Mentha arvensis extracts and characterized through UV-visible spectroscopy (peak absorbance at 295 nm), FTIR (13 functional group peaks acting as capping/stabilizing agents), SEM (spherical morphology, 164 nm average size), and zeta-potential (-32.9 mV). (RCBD, three replications) evaluated the combined A factorial experiment (RCBD, three replications) evaluated the combined effects on physico-morphological traits, antioxidant activities, and fatty acids compositions. The combined application of (⁶⁰Co) irradiation and Zn-Se NCs significantly enhanced plant height, leaf area, fruit pod number, and 100-seed weight in canola. The Zn-Se NCs treatments alone increased stem diameter and number of leaves/plants more significantly than irradiation and Zn-Se NCs combined effects. Treatment with ⁶⁰Co at Gy-20 and Zn-Se NCs at 75 and 100 mg/L increased Chl a, Chl b, and total chlorophyll content by 43%, 66%, and 46%, respectively. Plants exposed to irradiation and Zn-Se NCs had little impact on total carotenoid content, relative water content, and membrane stability index. Antioxidant enzymes activities rose significantly under combined irradiation (Gy-10) and Zn-Se NCs (75-100 mg/L) for SOD (79%), POD (208%) and CAT (119%) as compared to the control. At 50 mg/L, Zn-Se NCs boosted DPPH (31%, Gy-20) and ABTS (29%, Gy-10) in both Canola-Super8 and NARC-Sarson. Furthermore, (⁶⁰Co) irradiation and Zn-Se NCs substantially enhanced percentages of oil, protein, oleic acid and linolenic acid compared to control plants. Canola-Super8 seeds treated with Gy-30 irradiation significantly reduced glucosinolates to 13.15 μg/mol, addressing key anti-nutritional factors. These finding highlights the potential of cobalt-60 (⁶⁰Co) irradiation and Zn-Se NCs as sustainable tools for canola biofortification, offering a novel strategy to simultaneously boost crop productivity and seed quality.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04428-w.

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锌硒纳米复合材料的合成及其对甘蓝型油菜生理形态参数、抗氧化性和脂肪酸组成的影响

本文研究了钴-60 (60Co) γ辐照和锌-硒纳米复合材料对两个油菜品种的激效效应。以大蒜（Allium sativum）和薄荷（Mentha arvensis）提取物为原料合成了锌硒纳米复合材料（Zn-Se NCs），并通过紫外可见光谱（吸光度峰为295 nm）、红外光谱（13个功能基团峰为封盖/稳定剂）、扫描电镜（平均尺寸为164 nm的球形形貌）和ζ电位（-32.9 mV）对其进行了表征。（RCBD， 3个重复）评价了组合析因试验（RCBD， 3个重复）评价了对物理形态性状、抗氧化活性和脂肪酸组成的综合影响。（60Co）辐照与Zn-Se NCs联合施用显著提高了油菜的株高、叶面积、果荚数和百粒重。单独Zn-Se NCs处理比辐照和Zn-Se NCs联合处理更显著地增加了茎粗和叶/株数。60Co （Gy-20）和Zn-Se NCs（75和100 mg/L）处理使叶绿素含量分别提高了43%、66%和46%。辐照和Zn-Se NCs对植物总类胡萝卜素含量、相对含水量和膜稳定性指标影响不大。在Gy-10和Zn-Se NCs （75 ~ 100 mg/L）联合照射下，SOD（79%）、POD（208%）和CAT（119%）的抗氧化酶活性显著高于对照。50mg /L Zn-Se NCs提高了油菜- super8和NARC-Sarson的DPPH （31%, Gy-20）和ABTS （29%, Gy-10）。此外，（60Co）辐照和Zn-Se NCs显著提高了对照植株的油脂、蛋白质、油酸和亚麻酸的百分比。Gy-30辐照处理的油菜籽硫代葡萄糖苷含量显著降低至13.15 μg/mol，解决了关键的抗营养因子问题。这些发现突出了钴-60 （60Co）辐照和锌- se NCs作为油菜生物强化可持续工具的潜力，提供了一种同时提高作物生产力和种子质量的新策略。补充信息：在线版本包含补充资料，提供地址：10.1007/s13205-025-04428-w。

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来源期刊

3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

6.00

自引率

0.00%

发文量

314

期刊介绍： 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.