Antimicrobial and Antioxidant Electrospun Membrane Incorporating Silver and Copper Complexes of Phenylboronic Acid-Functionalized 4,5-Diazafluorene Ligand.

IF 3.6 3区 医学 Q2 CHEMISTRY, MEDICINAL
Seçil Kaya, Caner Cebeci, Hande Hançer, Buşra Akgül, Ibrahim Erden, Tülin Özbek, Emrah Şefik Abamor, Serap Acar
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Abstract

Thermally crosslinked polyacrylic acid (PAA)-polyvinyl alcohol (PVA) nanofibrous (NFC) membranes were developed via electrospinning for potential wound dressing applications with antioxidant and antimicrobial properties. Silver and copper complexes (AgSK and CuSK) of a phenylboronic acid-functionalized 4,5-diazafluorene ligand (SK) were synthesized, characterized, and incorporated into the membranes. SEM imaging revealed uniform, bead-free nanofibers with an average diameter of 725.35 ± 173.79 nm. FTIR analysis confirmed the successful incorporation of compounds through characteristic C═N stretching bands at 1620-1630 cm-1. DPPH assay showed that SK, AgSK, and CuSK exhibited antioxidant activities of 54.6 ± 2.5%, 88.4 ± 3.5%, and 79.6 ± 3.4% at 90 min, respectively. Incorporation into the nanofibers did not significantly alter antioxidant performance. Antimicrobial activity was assessed against Escherichia coli, Staphylococcus aureus, and Candida albicans. AgSK showed the strongest inhibition, with MIC values of 62.5, 31.25, and 125 µg/mL, respectively. AgSK-loaded membranes (AgSK-NFC) demonstrate antimicrobial activity with > 98% reduction in all tested strains. Biocompatibility tests with L929 fibroblasts showed improved cell viability upon membrane incorporation. The membranes also exhibited high swelling capacity, good water resistance, and sustained release over 7 days. With this study, for the first time, the use of 4,5-diazafluorene and its metal complexes in nanofibrous membranes has been demonstrated. Thus, an interdisciplinary approach combining materials science and coordination chemistry is established, broadening the scope for future developments in nanofiber-based technologies.

苯基硼酸功能化4,5-重氮芴配体银铜配合物电纺丝抗菌抗氧化膜。
采用静电纺丝技术制备了具有抗氧化和抗菌性能的热交联聚丙烯酸(PAA)-聚乙烯醇(PVA)纳米纤维(NFC)膜。合成了一种苯基硼酸功能化4,5-二氮芴配体(SK)的银和铜配合物(AgSK和CuSK),对其进行了表征,并将其掺入到膜中。扫描电镜成像显示均匀、无珠的纳米纤维,平均直径为725.35±173.79 nm。FTIR分析通过1620-1630 cm-1的特征C = N拉伸带证实了化合物的成功掺入。DPPH实验显示,90 min时,SK、AgSK和CuSK的抗氧化活性分别为54.6±2.5%、88.4±3.5%和79.6±3.4%。纳米纤维的掺入对抗氧化性能没有显著影响。对大肠杆菌、金黄色葡萄球菌和白色念珠菌的抗菌活性进行了评估。AgSK的抑制作用最强,MIC值分别为62.5、31.25和125µg/mL。负载agsk的膜(AgSK-NFC)显示出抗菌活性,在所有测试菌株中降低bb0 98%。L929成纤维细胞的生物相容性试验显示膜掺入后细胞活力提高。该膜还具有较高的溶胀能力,良好的耐水性和7天以上的持续释放。本研究首次证实了4,5-重氮芴及其金属配合物在纳米纤维膜中的应用。因此,将材料科学和配位化学相结合的跨学科方法被建立起来,扩大了纳米纤维技术未来发展的范围。
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来源期刊
Archiv der Pharmazie
Archiv der Pharmazie 医学-化学综合
CiteScore
7.90
自引率
5.90%
发文量
176
审稿时长
3.0 months
期刊介绍: Archiv der Pharmazie - Chemistry in Life Sciences is an international journal devoted to research and development in all fields of pharmaceutical and medicinal chemistry. Emphasis is put on papers combining synthetic organic chemistry, structural biology, molecular modelling, bioorganic chemistry, natural products chemistry, biochemistry or analytical methods with pharmaceutical or medicinal aspects such as biological activity. The focus of this journal is put on original research papers, but other scientifically valuable contributions (e.g. reviews, minireviews, highlights, symposia contributions, discussions, and essays) are also welcome.
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