PCR-RFLP assays to detect recessive lethal alleles in Landrace and Duroc pigs in Vietnam.

IF 1.1 3区农林科学 Q3 VETERINARY SCIENCES

Journal of Veterinary Diagnostic Investigation Pub Date : 2025-09-01 Epub Date: 2025-08-07 DOI:10.1177/10406387251318022

Anh Phu Nam Bui, Nghiep Mai Nguyen, Quang Le

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引用次数: 0

Abstract

Increasing the number of live-born piglets per litter is one of the major objectives of pig breeding programs. Single nucleotide polymorphisms (SNPs) in polynucleotide kinase-phosphatase (PNKP), RNA polymerase I subunit B (POLR1B), ribosome biogenesis (URB1), and transcriptional adaptors ADA2 (TADA2A genes are recessive lethal alleles that are associated with embryonic lethality, which affects the reproductive performance and animal welfare of pig populations). No assay is available to screen for the recessive haplotypes of these 4 SNPs in pig populations. We successfully developed and verified a rapid, simple, and cost-effective PCR-restriction-fragment length polymorphism (PCR-RFLP) assay for genotyping SNPs in PNKP, POLR1B, URB1 genes in Landrace and TADA2A genes in Duroc pigs in Vietnam. Our PCR-RFLP approach could potentially be applied in screening programs on a larger scale to improve the reproductive performance of commercial pig populations.

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PCR-RFLP检测越南长白猪和杜洛克猪隐性致死等位基因。

增加每窝活产仔猪的数量是猪育种计划的主要目标之一。多核苷酸激酶-磷酸酶（PNKP）、RNA聚合酶I亚基B （POLR1B）、核糖体生物发生（URB1）和转录接子ADA2 （TADA2A基因是与胚胎致病性相关的隐性致死等位基因，影响猪群体的繁殖性能和动物福利）的单核苷酸多态性（snp）。在猪群体中，没有检测方法可以筛选这4个snp的隐性单倍型。我们成功开发并验证了一种快速、简单、经济高效的pcr -限制性片段长度多态性（PCR-RFLP）方法，用于对越南杜罗克猪的PNKP、POLR1B、URB1基因和TADA2A基因的snp进行基因分型。我们的PCR-RFLP方法可能应用于更大规模的筛选程序，以提高商品猪群体的繁殖性能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Veterinary Diagnostic Investigation 农林科学-兽医学

CiteScore

3.00

自引率

6.70%

发文量

127

审稿时长

6-16 weeks

期刊介绍： The Journal of Veterinary Diagnostic Investigation (J Vet Diagn Invest) is an international peer-reviewed journal published bimonthly in English by the American Association of Veterinary Laboratory Diagnosticians (AAVLD). JVDI is devoted to all aspects of veterinary laboratory diagnostic science including the major disciplines of anatomic pathology, bacteriology/mycology, clinical pathology, epidemiology, immunology, laboratory information management, molecular biology, parasitology, public health, toxicology, and virology.