Taurine attenuates LPS-induced pro-inflammatory cytokine production in IPEC-J2 cells by inhibiting the TLR4-MyD88/TRIF signaling pathway.

IF 3.5 3区 医学 Q3 IMMUNOLOGY
Microbial pathogenesis Pub Date : 2025-11-01 Epub Date: 2025-08-09 DOI:10.1016/j.micpath.2025.107961
Shumei Lin, Xinxin Wang, Yifan Rao, Yuwen Song, Shuang Gao, Tiange Wang, Pengxian Wang, Zijun Qi, Yuxin Wang, Dongdong Zhao, Jiancheng Yang, Jianmin Hu
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引用次数: 0

Abstract

Post-weaning diarrhea (PWD) in piglets poses a significant challenge to global swine production, primarily due to weaning stress leading to intestinal barrier dysfunction. Recent studies have identified taurine, a conditionally essential amino acid, as a crucial modulator of innate immune responses. In this study, we utilized lipopolysaccharide (LPS)-treated porcine intestinal epithelial cells (IPEC-J2) to investigate the anti-inflammatory mechanisms of taurine. Pretreatment with taurine (20 mM for 48 h) significantly reduced the LPS-induced upregulation of pro-inflammatory cytokines, including IL-1β, TNF-α, IL-6, and IFN-β (p < 0.01). Immunofluorescence, Western blotting, and qRT-PCR analyses revealed that taurine suppressed Toll-like receptor 4 (TLR4) expression, inhibited the nuclear translocation of NF-κB p65, and attenuated the phosphorylation of MAPK (p38, ERK, JNK) and IRF3. These findings indicate that taurine mitigates inflammatory cascades by targeting the TLR4-MyD88/TRIF signaling pathway, thereby protecting IPEC-J2 cells from LPS-induced damage. This study elucidates the molecular mechanisms underlying taurine's anti-inflammatory effects and supports its potential role as a nutritional immunomodulator in managing PWD.

牛磺酸通过抑制TLR4-MyD88/TRIF信号通路减弱lps诱导的IPEC-J2细胞中促炎细胞因子的产生
仔猪断奶后腹泻(PWD)对全球猪生产构成重大挑战,主要是由于断奶应激导致肠道屏障功能障碍。最近的研究发现牛磺酸是一种条件必需氨基酸,是先天免疫反应的重要调节剂。在本研究中,我们利用脂多糖(LPS)处理的猪肠上皮细胞(IPEC-J2)来研究牛磺酸的抗炎机制。牛磺酸预处理(20 mM, 48 h)显著降低lps诱导的促炎细胞因子上调,包括IL-1β、TNF-α、IL-6和IFN-β (p
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来源期刊
Microbial pathogenesis
Microbial pathogenesis 医学-免疫学
CiteScore
7.40
自引率
2.60%
发文量
472
审稿时长
56 days
期刊介绍: Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)
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