Isolation, whole-genome analysis, and anti-biofilm activity of carbapenem-resistant Enterobacteriaceae phage KP-BW9 and its lysin Lys-BW9.

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-11-01 Epub Date: 2025-08-05 DOI:10.1016/j.micpath.2025.107960

Qingling Liu, Xiangyu Cao, Mengge Chen, Xin Yue, Longlong Liu, Rong Yang, Bo Xu, Hongkuan Deng

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引用次数: 0

Abstract

Carbapenem-resistant Enterobacteriaceae (CRE) have been classified as "urgent priority pathogens" due to their high drug resistance and wide spread. Among them, carbapenem-resistant Escherichia coli (CREco) and carbapenem-resistant Klebsiella pneumoniae (CRKP) are the main types. Phages are naturally occurring viruses that can specifically infect and lyse host bacteria, making them an effective alternative to antibiotics. This study isolated and identified a CREco phage, KP-BW9. The sequencing of phage KP-BW9 showed a genome size of 59665 bp, a GC content of 56.67 %, and encoded 79 predicted proteins. It has a latent period and a lysis period, with strong adaptability to the environment, and a lysis rate of 41.67 % for CREco. Both phage KP-BW9 and lysin Lys-BW9 could effectively lyse Escherichia coli and Klebsiella pneumoniae, and significantly inhibit and eradicate the biofilm of their individual and mixed bacteria. As a novel anti-CRE phage, KP-BW9 offers an innovative solution for controlling multidrug-resistant bacterial infections.

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耐碳青霉烯肠杆菌科噬菌体KP-BW9及其溶酶体Lys-BW9的分离、全基因组分析及抗生物膜活性研究

耐碳青霉烯肠杆菌科（CRE）因其高耐药性和广泛传播而被列为“紧急重点病原体”。其中，耐碳青霉烯型大肠杆菌（CREco）和耐碳青霉烯型肺炎克雷伯菌（CRKP）是主要类型。噬菌体是自然产生的病毒，可以特异性地感染和溶解宿主细菌，使其成为抗生素的有效替代品。本研究分离鉴定了CREco噬菌体KP-BW9。噬菌体KP-BW9测序结果显示，该噬菌体基因组大小为59665 bp， GC含量为56.67%，编码79种预测蛋白。具有潜伏期和裂解期，对环境适应性强，对CREco的裂解率为41.67%。噬菌体KP-BW9和溶酶Lys-BW9均能有效地裂解大肠埃希菌和肺炎克雷伯菌，并能显著抑制和根除其单独和混合菌的生物膜。作为一种新型抗cre噬菌体，KP-BW9为控制多重耐药细菌感染提供了一种创新的解决方案。

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)