Decoding JFT: a multifunctional fluorescence probe for sulfite and viscosity insights.

IF 4.2 3区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Frontiers in Chemistry Pub Date : 2025-07-23 eCollection Date: 2025-01-01 DOI:10.3389/fchem.2025.1642191

Hua Han, Bin Han, Yongjin Peng, Yuling Liu

引用次数: 0

Abstract

This study focuses on a multifunctional fluorescence probe JFT based on the FRET (Fluorescence Resonance Energy Transfer) and TICT (Twisted Intramolecular Charge Transfer) mechanism. JFT combines an electron donor and an acceptor, enabling it to detect sulfite and monitor intracellular viscosity. When reacting with sulfite, its electronic structure changes, turning off FRET and altering fluorescence wavelength and intensity. In different viscosity environments, the rotation of carbon-carbon bonds in the electron acceptor structure of JFT varies, affecting non-radiative transition pathways and fluorescence intensity. Theoretical calculations based on TDDFT reveal the electron distribution changes before and after the reaction with sulfite species, consistent with experimental phenomena. These findings deepen the understanding of the FRET mechanism of fluorescence probes and offer theoretical guidance for the design of more efficient fluorescence probes.

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解码JFT：一个多功能荧光探针亚硫酸盐和粘度的见解。

本文研究了一种基于荧光共振能量转移（FRET）和扭曲分子内电荷转移（TICT）机制的多功能荧光探针JFT。JFT结合了电子供体和受体，使其能够检测亚硫酸盐和监测细胞内粘度。当与亚硫酸盐反应时，其电子结构发生变化，关闭FRET，改变荧光波长和强度。在不同的粘度环境下，JFT电子受体结构中碳-碳键的旋转会发生变化，影响非辐射跃迁途径和荧光强度。基于TDDFT的理论计算揭示了与亚硫酸盐反应前后电子分布的变化，与实验现象一致。这些发现加深了对荧光探针FRET机制的理解，为设计更高效的荧光探针提供了理论指导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Chemistry Chemistry-General Chemistry

CiteScore

8.50

自引率

3.60%

发文量

1540

审稿时长

12 weeks

期刊介绍： Frontiers in Chemistry is a high visiblity and quality journal, publishing rigorously peer-reviewed research across the chemical sciences. Field Chief Editor Steve Suib at the University of Connecticut is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to academics, industry leaders and the public worldwide. Chemistry is a branch of science that is linked to all other main fields of research. The omnipresence of Chemistry is apparent in our everyday lives from the electronic devices that we all use to communicate, to foods we eat, to our health and well-being, to the different forms of energy that we use. While there are many subtopics and specialties of Chemistry, the fundamental link in all these areas is how atoms, ions, and molecules come together and come apart in what some have come to call the “dance of life”. All specialty sections of Frontiers in Chemistry are open-access with the goal of publishing outstanding research publications, review articles, commentaries, and ideas about various aspects of Chemistry. The past forms of publication often have specific subdisciplines, most commonly of analytical, inorganic, organic and physical chemistries, but these days those lines and boxes are quite blurry and the silos of those disciplines appear to be eroding. Chemistry is important to both fundamental and applied areas of research and manufacturing, and indeed the outlines of academic versus industrial research are also often artificial. Collaborative research across all specialty areas of Chemistry is highly encouraged and supported as we move forward. These are exciting times and the field of Chemistry is an important and significant contributor to our collective knowledge.