{"title":"Gene Expression Analysis of Early Stage Changes in Pancreatic Cancer by <i>Kras<sup>G12D</sup></i> Transfer in Pancreatic Progenitor-Like Cells.","authors":"Eiji Yamato","doi":"10.1080/07357907.2025.2543849","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study is to elucidate the function of <i>Kras</i>, which is involved in the development and progression of pancreatic cancer.</p><p><strong>Methods: </strong>We have previously isolated pancreas-derived cells from mice and induced genes expressed in pancreatic ducts and exocrine and endocrine cells by expressing transcription factor genes and a plasmid expressing polyoma ori to create cells with stable foreign gene expression. Comprehensive gene analysis was used to analyze the function of <i>Kras</i> by introducing wild-type <i>Kras</i> and mutant <i>Kras</i> into these cells.</p><p><strong>Results: </strong>No changes in cell morphology were observed with wild-type Kras expression, but expression of mutant <i>Kras</i> led to cystic changes. Mutant <i>Kras</i> gene transfer induced the expression of <i>Mus5ac</i> and <i>Cck</i> genes. Gene expression in these cells was detected by microarray analysis, and comparison of gene profiles showed that the genes promoted amoeboid cell assembly and immunosuppression.</p><p><strong>Conclusion: </strong>The use of these cells will facilitate screening of drugs that block <i>Kras</i> function and elucidate how <i>Kras</i> induces pancreatic cancer.</p>","PeriodicalId":9463,"journal":{"name":"Cancer Investigation","volume":" ","pages":"467-476"},"PeriodicalIF":1.9000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cancer Investigation","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/07357907.2025.2543849","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/6 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ONCOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: The aim of this study is to elucidate the function of Kras, which is involved in the development and progression of pancreatic cancer.
Methods: We have previously isolated pancreas-derived cells from mice and induced genes expressed in pancreatic ducts and exocrine and endocrine cells by expressing transcription factor genes and a plasmid expressing polyoma ori to create cells with stable foreign gene expression. Comprehensive gene analysis was used to analyze the function of Kras by introducing wild-type Kras and mutant Kras into these cells.
Results: No changes in cell morphology were observed with wild-type Kras expression, but expression of mutant Kras led to cystic changes. Mutant Kras gene transfer induced the expression of Mus5ac and Cck genes. Gene expression in these cells was detected by microarray analysis, and comparison of gene profiles showed that the genes promoted amoeboid cell assembly and immunosuppression.
Conclusion: The use of these cells will facilitate screening of drugs that block Kras function and elucidate how Kras induces pancreatic cancer.
期刊介绍：
Cancer Investigation is one of the most highly regarded and recognized journals in the field of basic and clinical oncology. It is designed to give physicians a comprehensive resource on the current state of progress in the cancer field as well as a broad background of reliable information necessary for effective decision making. In addition to presenting original papers of fundamental significance, it also publishes reviews, essays, specialized presentations of controversies, considerations of new technologies and their applications to specific laboratory problems, discussions of public issues, miniseries on major topics, new and experimental drugs and therapies, and an innovative letters to the editor section. One of the unique features of the journal is its departmentalized editorial sections reporting on more than 30 subject categories covering the broad spectrum of specialized areas that together comprise the field of oncology. Edited by leading physicians and research scientists, these sections make Cancer Investigation the prime resource for clinicians seeking to make sense of the sometimes-overwhelming amount of information available throughout the field. In addition to its peer-reviewed clinical research, the journal also features translational studies that bridge the gap between the laboratory and the clinic.
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