Beauveria bassiana as a versatile biocatalyst: advances in regioselective hydroxylation and applications in biological control.

Abstract

Beauveria bassiana exhibits remarkable biocatalytic versatility in regioselective hydroxylation, enabling efficient functionalization of chemically inert substrates. This fungus demonstrates high regio-selectivity and stereoselectivity across diverse compounds, including aliphatic, aromatic, and heterocyclic substrates. Notably, B. bassiana has demonstrated key hydroxylation achievements, efficiently hydroxylating compounds, such as stemodin and stemodinone, to produce intermediates for essential drugs with yields of up to 53%. Strain optimization via CRISPR/Cas9 editing and adaptive evolution has expanded substrate scope and improved yields, while organic solvent supplementation enhanced biocatalytic efficiency. We emphasize its genomic and enzymatic foundations, industrial applications, and role in insect pest control through virulence-linked hydroxylation pathways. For instance, B. bassiana has been successfully employed in the biological control of various pests, including lepidopteran and coleopteran species. However, challenges, such as non-target organism impacts and resistance development, necessitate integrated strategies, including targeted delivery systems and combinatorial biocontrol approaches. Industrial applications leverage B. bassiana for synthesizing drug intermediates and bioactive metabolites with enhanced aromatase inhibition. Future directions include elucidating novel hydroxylases, refining genetic engineering for host specificity, and optimizing fermentation processes to bridge bio-catalysis with sustainable agriculture and green chemistry.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04456-6.