A mitochondrial-targeted fluorescent probe for imaging HOCl in ferroptosis and multiple liver injury models

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Analytical and Bioanalytical Chemistry Pub Date : 2025-08-05 DOI:10.1007/s00216-025-06028-2

Cailing Fan, Na Xiao, Yixing Shi, Yonghao Song, Lei Liu, Yuwei Wu, Keying Yang, Yongwei Limeng, Qinxi Dong, Yanan Gao, Wei Shu, Chaoyuan Zeng

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引用次数: 0

Abstract

Ferroptosis exerts a significant influence on the pathogenesis, progression, and therapeutic outcomes of liver diseases. Mitochondria play pivotal roles in liver disease and are also an indispensable hub in the final implementation of ferroptosis. The overabundance of mitochondrial HOCl can result in the permeabilization of mitochondria, which can cause cell death and damage to liver tissue. Herein, we designed a fluorescent probe named PTZ-PY based on thiophenazine for HOCl rapid detection. PTZ-PY exhibits excellent sensitivity and high selectivity to HOCl, and the LOD of HOCl is 13 nM. PTZ-PY is capable of targeting mitochondria and effectively monitors fluctuations in endogenous HOCl concentrations within cellular and zebrafish models, and has been utilized to document the escalation of HOCl levels associated with ferroptosis. As an efficient tool for detecting HOCl, PTZ-PY has demonstrated remarkable potential in exploring functions of HOCl in physiological processes, suggesting that it holds a promising application prospect in related research fields.

Graphical Abstract

Abstract Image

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线粒体靶向荧光探针在铁下垂和多发性肝损伤模型中成像HOCl。

铁下垂对肝脏疾病的发病、进展和治疗结果有重要影响。线粒体在肝脏疾病中起着关键作用，也是最终实现铁下垂不可或缺的枢纽。线粒体HOCl含量过高可导致线粒体通透，从而导致细胞死亡和肝组织损伤。为此，我们设计了一种基于噻吩嗪的荧光探针PTZ-PY用于HOCl的快速检测。PTZ-PY对HOCl具有良好的灵敏度和选择性，HOCl的LOD为13 nM。PTZ-PY能够靶向线粒体，并有效监测细胞和斑马鱼模型中内源性HOCl浓度的波动，并已用于记录与铁下垂相关的HOCl水平的升高。PTZ-PY作为一种检测HOCl的有效工具，在探索HOCl在生理过程中的功能方面显示出显著的潜力，在相关研究领域具有广阔的应用前景。

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来源期刊

Analytical and Bioanalytical Chemistry 化学-分析化学

CiteScore

8.00

自引率

4.70%

发文量

638

审稿时长

2.1 months

期刊介绍： Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.