HOXC6 Activates BCAT1 Expression to Promote Oral Cancer Development.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2025-08-05 DOI:10.1111/odi.70049

Meiheriban Tuerhong, Zaynure Wubulihasimu, Bo Xu, Ling Zhang

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引用次数: 0

Abstract

Objective: This paper aims to reveal the impact of HOXC6 on oral cancer development through the regulation of BCAT1 and the molecular mechanism.

Methods: BCAT1 expression in oral cancer tissues and adjacent tissues was verified by western blot analysis and RT-qPCR. After knocking down BCAT1, the HN6 and HN30 cell proliferation, migration, invasion, and apoptosis were detected. The upstream mechanism of BCAT1 elevation was investigated by bioinformatics analysis. HOXC6 expression was detected by immunohistochemistry in oral cancer and adjacent tissues. ChIP and dual-luciferase assay were used to detect the interaction between HOXC6 and the BCAT1 promoter. Rescue experiments were carried out to substantiate the malignant phenotype and epithelial-to-mesenchymal transition (EMT) of oral cancer cells. An in vivo xenograft tumor model was constructed.

Results: BCAT1 and HOXC6 were abnormally high in oral cancer tissues. Inhibition of BCAT1 suppressed the proliferation, migration, invasion, and EMT, and promoted apoptosis of oral cancer cells. HOXC6 downregulation curbed the malignant behavior and EMT program of oral cancer cells. HOXC6 was enriched in the BCAT1 promoter in oral cancer cells. HOXC6 silencing downregulated BCAT1 expression. Transcriptional activation of BCAT1 by HOXC6 promoted oral cancer progression.

Conclusion: HOXC6 knockdown inhibits BCAT1 expression to suppress oral cancer development.

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HOXC6激活BCAT1表达促进口腔癌发展

目的：本文旨在通过BCAT1的调控揭示HOXC6对口腔癌发展的影响及其分子机制。方法：采用western blot和RT-qPCR检测BCAT1在口腔癌组织及癌旁组织中的表达。敲除BCAT1后，检测HN6和HN30细胞的增殖、迁移、侵袭和凋亡。通过生物信息学分析探讨BCAT1升高的上游机制。免疫组化检测HOXC6在口腔癌及癌旁组织中的表达。采用ChIP和双荧光素酶法检测HOXC6与BCAT1启动子的相互作用。通过挽救实验证实了口腔癌细胞的恶性表型和上皮-间质转化（EMT）。建立了活体异种移植瘤模型。结果：BCAT1、HOXC6在口腔癌组织中表达异常高。抑制BCAT1抑制口腔癌细胞的增殖、迁移、侵袭和EMT，促进细胞凋亡。HOXC6下调抑制口腔癌细胞的恶性行为和EMT程序。HOXC6在口腔癌细胞BCAT1启动子中富集。HOXC6沉默下调的BCAT1表达。HOXC6对BCAT1的转录激活促进口腔癌的进展。结论：HOXC6敲低可抑制BCAT1的表达，抑制口腔癌的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.