Daughter Cells Budding From PGCCs and Their Clinicopathological Significances in Oral Squamous Cell Carcinoma

IF 2.3 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Pathology & Medicine Pub Date : 2025-08-05 DOI:10.1111/jop.70014

Shanshan Zhuo, Chao Jing, Xiaonan Liu, Jiuling Yue, Wenchao Zhang, Shiwu Zhang

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引用次数: 0

Abstract

Objective

Polyploid giant cancer cells (PGCCs) have emerged as a focal point in tumorigenesis and progression research. Present across various tumor tissues, PGCCs are considered a potential target for anti-tumor strategies. Nonetheless, their presence and role in oral squamous cell carcinoma (OSCC) remain undocumented. This study investigated the presence and count of PGCCs in OSCC tissues, evaluated their association with clinicopathological factors, and preliminarily examined the mechanisms underlying their formation alongside their influence on OSCC proliferation, drug resistance, and migratory capacity.

Methods

The correlation between PGCC counts and clinical outcomes in OSCC was evaluated. CoCl₂ treatment was used to induce PGCC formation in OSCCUM1 cells, and alterations in HIF-1α, SOX-2, E-cadherin, and N-cadherin expression, along with changes in cellular proliferation, drug resistance, and migratory capacity, were assessed.

Results

Among 76 OSCC patients, PGCC counts were linked to clinical stage, histological grade, and chemotherapy response. Patients with > 3 PGCCs/HP exhibited significantly poorer overall and disease-free survival compared to those with ≤ 3 PGCCs/HP. Following the induction of polyploid giant cancer cells with budding cells, miRNA analysis revealed upregulated SOX-2 expression, while Western blot demonstrated increased protein levels of HIF-1α, SOX-2, and N-cadherin, accompanied by reduced E-cadherin expression. Induced PGCCs with budding cells also exhibited enhanced proliferation, resistance to chemotherapy, and migratory capacity.

Conclusion

Hypoxic conditions may promote OSCC proliferation, chemoresistance, and migration through the formation of PGCCs with budding cells characterized by epithelial-mesenchymal transition and stemness phenotypes.

查看原文本刊更多论文

口腔鳞状细胞癌中pgcc出芽的子细胞及其临床病理意义。

目的：多倍体巨细胞（Polyploid giant cancer cells, PGCCs）已成为肿瘤发生发展研究的热点。存在于多种肿瘤组织中，pgcc被认为是抗肿瘤策略的潜在靶点。尽管如此，它们在口腔鳞状细胞癌（OSCC）中的存在和作用仍未得到证实。本研究调查了鳞癌组织中pgcc的存在和计数，评估了它们与临床病理因素的关系，并初步探讨了它们形成的机制以及它们对鳞癌增殖、耐药性和迁移能力的影响。方法：评价鳞癌患者PGCC计数与临床预后的相关性。使用CoCl2处理诱导OSCCUM1细胞形成PGCC，并评估HIF-1α、SOX-2、E-cadherin和N-cadherin表达的变化，以及细胞增殖、耐药性和迁移能力的变化。结果：在76例OSCC患者中，PGCC计数与临床分期、组织学分级和化疗反应有关。与≤3 PGCCs/HP的患者相比，bbb3 PGCCs/HP患者的总生存期和无病生存期明显较差。在诱导多倍体巨细胞出芽后，miRNA分析显示SOX-2表达上调，而Western blot显示HIF-1α、SOX-2和N-cadherin蛋白水平升高，同时E-cadherin表达降低。带出芽细胞的诱导pgcc也表现出增强的增殖、化疗抗性和迁移能力。结论：缺氧条件可能通过形成以上皮间质转化和干性表型为特征的出芽细胞pgcc，促进OSCC增殖、耐药和迁移。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Oral Pathology & Medicine 医学-病理学

CiteScore

5.90

自引率

6.10%

发文量

121

审稿时长

4-8 weeks

期刊介绍： The aim of the Journal of Oral Pathology & Medicine is to publish manuscripts of high scientific quality representing original clinical, diagnostic or experimental work in oral pathology and oral medicine. Papers advancing the science or practice of these disciplines will be welcomed, especially those which bring new knowledge and observations from the application of techniques within the spheres of light and electron microscopy, tissue and organ culture, immunology, histochemistry and immunocytochemistry, microbiology, genetics and biochemistry.