Very long-chain fatty acids accumulate in breast cancer tissue and serum.

IF 6 2区医学 Q1 ONCOLOGY

Cancer Cell International Pub Date : 2025-08-04 DOI:10.1186/s12935-025-03928-2

Alicja Pakiet, Michalina Ciosek, Oliwia Lange, Katarzyna Duzowska, Agata Janczy, Małgorzata Kapusta, Yelyzaveta Razghonova, Marcin Ekman, Anna Abacajew-Chmyłko, Paweł Kabata, Adriana Mika

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引用次数: 0

Abstract

Background: Breast cancer (BC) remains one of the most common cancers with relatively high mortality and is associated with alterations in fatty acid (FA) metabolism. While typical FAs have been extensively studied, there is increasing evidence for a potential role of very long chain fatty acids (VLCFAs) in cancer growth and progression.

Methods: This study involved 54 BC patients from whom samples of malignant tumor, normal fibroglandular tissue, and breast adipose tissue were collected. Their FA content was analyzed by gas chromatography-mass spectrometry. The expression of fatty acid elongases (ELOVLs) and FA-transporting proteins was analyzed in the tissues by RT-PCR and immunofluorescence.

Results: Higher levels of saturated and monounsaturated VLCFAs were found in BC tissues compared to normal tissues (p < 0.001) and in patients' blood compared to healthy controls blood (p < 0.001). However, the level of VLCFAs was lower in BC adipose tissue compared to healthy control adipose tissue (p < 0.001). Interestingly, there were no obvious differences in ELOVL1 mRNA or protein levels between normal and cancer tissues.

Conclusions: Elevated levels of VLCFAs in BC tissue are the result of lipid uptake from outside the tumor rather than in situ synthesis.

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长链脂肪酸在乳腺癌组织和血清中积累。

背景：乳腺癌（BC）仍然是最常见的癌症之一，死亡率相对较高，并与脂肪酸（FA）代谢的改变有关。虽然典型的脂肪酸已被广泛研究，但越来越多的证据表明，长链脂肪酸（VLCFAs）在癌症生长和进展中的潜在作用。方法：本研究共收集了54例BC患者的恶性肿瘤、正常纤维腺组织和乳腺脂肪组织样本。采用气相色谱-质谱联用分析了它们的FA含量。采用RT-PCR和免疫荧光法检测各组脂肪酸延长酶（ELOVLs）和fa转运蛋白的表达。结果：与正常组织相比，在BC组织中发现了更高水平的饱和和单不饱和VLCFAs (p)。结论：BC组织中VLCFAs水平升高是肿瘤外脂质摄取而不是原位合成的结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer Cell International ONCOLOGY-

CiteScore

10.90

自引率

1.70%

发文量

360

审稿时长

1 months

期刊介绍： Cancer Cell International publishes articles on all aspects of cancer cell biology, originating largely from, but not limited to, work using cell culture techniques. The journal focuses on novel cancer studies reporting data from biological experiments performed on cells grown in vitro, in two- or three-dimensional systems, and/or in vivo (animal experiments). These types of experiments have provided crucial data in many fields, from cell proliferation and transformation, to epithelial-mesenchymal interaction, to apoptosis, and host immune response to tumors. Cancer Cell International also considers articles that focus on novel technologies or novel pathways in molecular analysis and on epidemiological studies that may affect patient care, as well as articles reporting translational cancer research studies where in vitro discoveries are bridged to the clinic. As such, the journal is interested in laboratory and animal studies reporting on novel biomarkers of tumor progression and response to therapy and on their applicability to human cancers.