Sterilization of Stainless-Steel Surfaces Using Ultraviolet Radiation Produced by Light-Emitting Diodes.

IF 2.6 3区 物理与天体物理 Q2 ASTRONOMY & ASTROPHYSICS
Astrobiology Pub Date : 2025-08-01 Epub Date: 2025-08-05 DOI:10.1177/15311074251365196
Joseph Wood, Mariela Monge, Emily P Seto, Katherine Ratliff, Brian Ford, Denise Aslett, Ahmed Abdel-Hady, Lesley Mendez Sandoval
{"title":"Sterilization of Stainless-Steel Surfaces Using Ultraviolet Radiation Produced by Light-Emitting Diodes.","authors":"Joseph Wood, Mariela Monge, Emily P Seto, Katherine Ratliff, Brian Ford, Denise Aslett, Ahmed Abdel-Hady, Lesley Mendez Sandoval","doi":"10.1177/15311074251365196","DOIUrl":null,"url":null,"abstract":"<p><p>Numerous studies have demonstrated that ultraviolet radiation in the C wavelength range produced by light-emitting diodes (UVC-LEDs) is effective for disinfection (<i>i.e.,</i> inactivation of vegetative bacteria and viruses). However, there are few efficacy data available to confirm its use as a sterilization technique (complete inactivation of bacterial spores). The present study evaluated the use of UVC-LED to achieve the sterilization of stainless-steel surfaces as a function of UVC dose and several other variables. Spores of <i>Bacillus atrophaeus</i> and two strains of <i>Bacillus pumilus</i> were used as indicator microorganisms. Results showed that the microorganism, spore loading, and inoculation method all affected whether complete inactivation was achieved. Under the tested conditions, sterilization of stainless-steel surfaces was achieved using UV-LED with doses that ranged from ∼4500 to 21,000 mJ/cm<sup>2</sup>, and if spore deposition was low enough to prevent clumping and subsequent shielding. We found that spore deposition in which sterilization was achieved ranged from 2.9 to 6.2 log<sub>10</sub> colony-forming units/cm<sup>2</sup> and depended primarily on the microorganism/strain. Shielding of UV radiation diminished efficacy and may have also occurred from the presence of foreign material.</p>","PeriodicalId":8645,"journal":{"name":"Astrobiology","volume":" ","pages":"550-562"},"PeriodicalIF":2.6000,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Astrobiology","FirstCategoryId":"101","ListUrlMain":"https://doi.org/10.1177/15311074251365196","RegionNum":3,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/8/5 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"ASTRONOMY & ASTROPHYSICS","Score":null,"Total":0}
引用次数: 0

Abstract

Numerous studies have demonstrated that ultraviolet radiation in the C wavelength range produced by light-emitting diodes (UVC-LEDs) is effective for disinfection (i.e., inactivation of vegetative bacteria and viruses). However, there are few efficacy data available to confirm its use as a sterilization technique (complete inactivation of bacterial spores). The present study evaluated the use of UVC-LED to achieve the sterilization of stainless-steel surfaces as a function of UVC dose and several other variables. Spores of Bacillus atrophaeus and two strains of Bacillus pumilus were used as indicator microorganisms. Results showed that the microorganism, spore loading, and inoculation method all affected whether complete inactivation was achieved. Under the tested conditions, sterilization of stainless-steel surfaces was achieved using UV-LED with doses that ranged from ∼4500 to 21,000 mJ/cm2, and if spore deposition was low enough to prevent clumping and subsequent shielding. We found that spore deposition in which sterilization was achieved ranged from 2.9 to 6.2 log10 colony-forming units/cm2 and depended primarily on the microorganism/strain. Shielding of UV radiation diminished efficacy and may have also occurred from the presence of foreign material.

利用发光二极管产生的紫外线辐射对不锈钢表面进行灭菌。
大量研究表明,由发光二极管(uvc - led)产生的C波长范围的紫外线辐射对消毒(即灭活植物性细菌和病毒)有效。然而,很少有可用的功效数据来证实其作为灭菌技术(完全灭活细菌孢子)的使用。本研究评估了UVC- led实现不锈钢表面灭菌的作用,作为UVC剂量和其他几个变量的函数。以萎缩芽孢杆菌孢子和两株短小芽孢杆菌作为指示微生物。结果表明,微生物、孢子载量和接种方法均影响菌体能否完全失活。在测试条件下,使用UV-LED实现不锈钢表面的灭菌,剂量范围从~ 4500到21,000 mJ/cm2,如果孢子沉积足够低以防止结块和随后的屏蔽。我们发现,灭菌的孢子沉积在2.9至6.2 log10菌落形成单位/cm2之间,主要取决于微生物/菌株。紫外线辐射的屏蔽降低了功效,也可能是由于外来物质的存在。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Astrobiology
Astrobiology 生物-地球科学综合
CiteScore
7.70
自引率
11.90%
发文量
100
审稿时长
3 months
期刊介绍: Astrobiology is the most-cited peer-reviewed journal dedicated to the understanding of life''s origin, evolution, and distribution in the universe, with a focus on new findings and discoveries from interplanetary exploration and laboratory research. Astrobiology coverage includes: Astrophysics; Astropaleontology; Astroplanets; Bioastronomy; Cosmochemistry; Ecogenomics; Exobiology; Extremophiles; Geomicrobiology; Gravitational biology; Life detection technology; Meteoritics; Planetary geoscience; Planetary protection; Prebiotic chemistry; Space exploration technology; Terraforming
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信