Molecular identification and genotyping of Blastocystis in farmed Cattle, Goats, and Pigs from Zhejiang Province, China

IF 3.1 Q2 PARASITOLOGY

Food and Waterborne Parasitology Pub Date : 2025-08-05 DOI:10.1016/j.fawpar.2025.e00280

Wei Zhao , Lijie Sun , Yanbin Sun , Xinyi Fu , Shiyang Ma , Jiayin Zhang , Baolong Yan

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Abstract

Blastocystis is a genus of parasitic protozoa that parasitize/colonize humans and animals gastrointestinal tract. The current study performed a molecular survey of Blastocystis in farm cattle (Bos tarurs), goats (Capra hircus), and pigs (Susscrofa domestica) raised in different cities in Zhejiang Province of China to better understand the epidemiology of Blastocystis in the animals of this region. A total of 859 fresh fecal samples were collected from 265 cattle, 386 goats, and 208 pigs. All these samples were screened for the detection of Blastocystis by amplifying the small subunit ribosomal RNA (SSU rRNA) gene via PCR and Sanger sequencing. Of the 859 samples tested, 12.1 % (104) were positive for Blastocystis, with cattle showing a prevalence of 19.6 % (52/265), goats with 11.4 % (44/386), and pigs with 3.8 % (8/208). Eight different subtypes of Blastocystis were found: ST10 (n = 51), ST5 (n = 11), ST12 (n = 9), ST14 (n = 8), ST21 (n = 8), ST23 (n = 7), ST26 (n = 6), and ST4 (n = 4). Cattle carried seven (ST5, ST10, ST12, ST14, ST21, ST23, and ST26), whereas goats harbored eight (ST4, ST5, ST10, ST12, ST14, ST21, ST23, and ST26) subtypes. All pig-derived Blastocystis isolates belonged only to ST5. These results are significant as they indicate that cattle, goats, and pigs in Zhejiang Province, China, harbor various subtypes of Blastocystis, which enhances our understanding of the distribution of Blastocystis among these hosts in China.

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浙江省养殖牛、山羊和猪囊虫的分子鉴定和基因分型

囊虫是一种寄生于人和动物胃肠道的寄生原生动物。本研究对浙江省不同城市养殖的牛（Bos tarurs）、山羊（Capra hircus）和猪（Susscrofa domestica）的囊虫进行了分子调查，以更好地了解该地区动物囊虫的流行病学。共采集了265头牛、386头山羊和208头猪的859份新鲜粪便样本。通过PCR和Sanger测序扩增小亚单位核糖体RNA （SSU rRNA）基因，筛选囊胚。在检测的859份样本中，12.1%（104份）囊虫呈阳性，其中牛的患病率为19.6%(52/265)，山羊为11.4%(44/386)，猪为3.8%（8/208）。发现8种不同的囊虫亚型：ST10 （n = 51）、ST5 （n = 11）、ST12 （n = 9）、ST14 （n = 8）、ST21 （n = 8）、ST23 （n = 7）、ST26 （n = 6）、ST4 （n = 4）。牛携带7种（ST5、ST10、ST12、ST14、ST21、ST23和ST26）亚型，山羊携带8种（ST4、ST5、ST10、ST12、ST14、ST21、ST23和ST26）亚型。所有猪源性囊虫分离株仅属于ST5。这些结果表明，中国浙江省的牛、山羊和猪中存在多种囊虫亚型，这有助于我们了解囊虫在中国这些宿主中的分布情况。

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来源期刊

Food and Waterborne Parasitology Immunology and Microbiology-Parasitology

CiteScore

5.10

自引率

4.00%

发文量

审稿时长

13 weeks

期刊介绍： Food and Waterborne Parasitology publishes high quality papers containing original research findings, investigative reports, and scientific proceedings on parasites which are transmitted to humans via the consumption of food or water. The relevant parasites include protozoa, nematodes, cestodes and trematodes which are transmitted by food or water and capable of infecting humans. Pertinent food includes products of animal or plant origin which are domestic or wild, and consumed by humans. Animals and plants from both terrestrial and aquatic sources are included, as well as studies related to potable and other types of water which serve to harbor, perpetuate or disseminate food and waterborne parasites. Studies dealing with prevalence, transmission, epidemiology, risk assessment and mitigation, including control measures and test methodologies for parasites in food and water are of particular interest. Evidence of the emergence of such parasites and interactions among domestic animals, wildlife and humans are of interest. The impact of parasites on the health and welfare of humans is viewed as very important and within scope of the journal. Manuscripts with scientifically generated information on associations between food and waterborne parasitic diseases and lifestyle, culture and economies are also welcome. Studies involving animal experiments must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences.