Non-invasive, non-enzymatic, non-serodiagnostic, and home-detecting paper-based “abnormal UA alarm” for early diagnosis of UA associated diseases

IF 13 Q1 MATERIALS SCIENCE, PAPER & WOOD

Journal of Bioresources and Bioproducts Pub Date : 2025-08-01 DOI:10.1016/j.jobab.2025.06.002

Qian Zhang , Hui’e Jiang , Zhijian Li , Lijuan Chen , Fengqian Yang , Jiamin Zhang , Bo Zhang , Xinhua Liu

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引用次数: 0

Abstract

Uric acid (UA) level is a pivotal clinical human-health biomarker providing predictive feedback for multitudinous well-known kidney, cardiovascular and metabolic syndrome diseases. Off-the-shelf UA detection methods clinically rely on uricase suffer from limitations such as high costs, longstanding result acquisition, circumscribed testing locations, rigorous expertise requirements, and difficulty in home-detecting due to serum testing systems. Here, inspired by the pH-paper, a scaleable, rapid, non-invasive/-enzymatic/-serodiagnostic, and home-detecting “abnormal UA alarm” platform for UA detection in saliva was developed by strategically integrating the proposed paper-based fluorescent sensing-materials (NIFP-SM) with a user-orientated intelligent red-green-blue (RGB) analysis device. Therefore, NIFP-SM is nano-engineered through straightforward interfacial interactions of functional building blocks of on-demand naphthyl imide-derived fluorescent self-assembled micro-particles (NIFS) with lamellar structure and commercially-used filter paper. The NIFS possesses dominantly wide detection range (0–5 000 µmol/L) and high sensitivity (limit of detection = 0.91 µmol/L). Surprisingly, NIFS exhibited outstanding identifiability for uric acid even in the presence of 34 interferents, substantiating accurate detection-capability in intricate environments. Thus NIFP-SM equipped with NIFS resoundingly achieved efficient, rapid, and on-site visual detection of UA in saliva, urine-simulants, and foods. Further, the NIFP-SM-based automatic analysis platform integrated with an intelligent RGB analysis device was manufactured and enabled accurate quantitative, low-cost, non-invasive/-enzymatic/-serodiagnostic, rapid, home-detecting for UA, eliminating the need for costly equipment and specialized personnel and thereby facilitating early-warning detection of abnormal UA-levels associated diseases.

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无创、无酶、无血清诊断和家庭检测纸质“异常UA报警”早期诊断UA相关疾病

尿酸（UA）水平是一种关键的临床人类健康生物标志物，为许多众所周知的肾脏、心血管和代谢综合征疾病提供预测反馈。临床上依赖于尿酸酶的现成UA检测方法存在成本高、结果获取时间长、检测地点受限、专业知识要求严格以及由于血清检测系统而难以在家庭检测等局限性。在这里，受ph论文的启发，通过将所提出的基于纸张的荧光传感材料（NIFP-SM）与面向用户的智能红绿蓝（RGB）分析设备战略性地集成在一起，开发了一种可扩展、快速、无创/酶促/血清诊断和家庭检测的唾液UA检测“异常UA报警”平台。因此，NIFP-SM是通过按需萘酰亚胺衍生的荧光自组装微颗粒（NIFS）的功能构建块与层状结构和商用滤纸的直接界面相互作用进行纳米工程的。NIFS检测范围宽（0 ~ 5 000µmol/L），灵敏度高（检出限0.91µmol/L）。令人惊讶的是，即使在34种干扰物存在的情况下，NIFS对尿酸也表现出了出色的可识别性，证实了在复杂环境下的准确检测能力。因此，配备NIFS的NIFP-SM能够高效、快速、现场可视化地检测唾液、尿液模拟物和食物中的UA。此外，基于nifp - sm的自动分析平台集成了智能RGB分析设备，实现了准确定量、低成本、无创/酶/血清诊断、快速、家庭检测UA，消除了对昂贵设备和专业人员的需求，从而促进了异常UA水平相关疾病的早期预警检测。

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