SARS-CoV-2, influenza, HRSV and other respiratory pathogens during the post-COVID-19 era: Epidemic circulation in Italy in the 2023/2024 season

IF 4 3区医学 Q1 INFECTIOUS DISEASES

Journal of Infection and Public Health Pub Date : 2025-07-18 DOI:10.1016/j.jiph.2025.102905

Serena Marchi , Valentina Salvestroni , Bianca Maria Bocci , Giovanni Guarducci , Giovanna Milano , Anna Carmina De Francesco , Emanuele Montomoli , Giovanni Bova , Andrea Camarri , Ilaria Manini

{"title":"SARS-CoV-2, influenza, HRSV and other respiratory pathogens during the post-COVID-19 era: Epidemic circulation in Italy in the 2023/2024 season","authors":"Serena Marchi , Valentina Salvestroni , Bianca Maria Bocci , Giovanni Guarducci , Giovanna Milano , Anna Carmina De Francesco , Emanuele Montomoli , Giovanni Bova , Andrea Camarri , Ilaria Manini","doi":"10.1016/j.jiph.2025.102905","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed at describing the epidemiological aspects of respiratory pathogens involved in cases of severe acute respiratory infections (SARI) in Siena (Tuscany, Italy) during the 2023/2024 season.</div></div><div><h3>Methods</h3><div>Oropharyngeal swabs were collected from enrolled patients in the University Hospital Trust of Siena. Swabs were tested by qRT-PCR for the detection of SARS-CoV-2 and other 21 respiratory pathogens, including influenza and human respiratory syncytial virus (HRSV). Swabs positive for SARS-CoV-2 and/or influenza were further analyzed by Next-Generation sequencing (NGS).</div></div><div><h3>Results</h3><div>From January to June 2024, 138 patients diagnosed with SARI were enrolled, with an average age of 76.7 years and a frequent presence of comorbidities. Among the patients, 40 % tested positive for SARS-CoV-2, followed by 20 % positivity for influenza and 13 % positivity for HRSV. For SARS-CoV-2, variants belonging to the JN.1, EG.5 and BA.5 lineages were found. The influenza A(H1N1)pdm09 strains were found to belong to the 6B.1a.5a.2a clade, while for influenza A(H3N2), the strains belonged to two different clades (3 C.2a1b.2a.1a and 3 C.2a1b. 2a.2a.3a.1).</div></div><div><h3>Conclusions</h3><div>This study contributes to the understanding of the spread of respiratory viruses and the genetic characteristics of SARS-CoV-2 and influenza in patients with SARI, underlining the importance of continuous surveillance.</div></div>","PeriodicalId":16087,"journal":{"name":"Journal of Infection and Public Health","volume":"18 11","pages":"Article 102905"},"PeriodicalIF":4.0000,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Infection and Public Health","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1876034125002540","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives

This study aimed at describing the epidemiological aspects of respiratory pathogens involved in cases of severe acute respiratory infections (SARI) in Siena (Tuscany, Italy) during the 2023/2024 season.

Methods

Oropharyngeal swabs were collected from enrolled patients in the University Hospital Trust of Siena. Swabs were tested by qRT-PCR for the detection of SARS-CoV-2 and other 21 respiratory pathogens, including influenza and human respiratory syncytial virus (HRSV). Swabs positive for SARS-CoV-2 and/or influenza were further analyzed by Next-Generation sequencing (NGS).

Results

From January to June 2024, 138 patients diagnosed with SARI were enrolled, with an average age of 76.7 years and a frequent presence of comorbidities. Among the patients, 40 % tested positive for SARS-CoV-2, followed by 20 % positivity for influenza and 13 % positivity for HRSV. For SARS-CoV-2, variants belonging to the JN.1, EG.5 and BA.5 lineages were found. The influenza A(H1N1)pdm09 strains were found to belong to the 6B.1a.5a.2a clade, while for influenza A(H3N2), the strains belonged to two different clades (3 C.2a1b.2a.1a and 3 C.2a1b. 2a.2a.3a.1).

Conclusions

This study contributes to the understanding of the spread of respiratory viruses and the genetic characteristics of SARS-CoV-2 and influenza in patients with SARI, underlining the importance of continuous surveillance.

查看原文本刊更多论文

后covid -19时代的SARS-CoV-2、流感、HRSV和其他呼吸道病原体：2023/2024季节意大利的流行病传播

目的本研究旨在描述2023/2024年意大利托斯卡纳锡耶纳严重急性呼吸道感染（SARI）病例中呼吸道病原体的流行病学方面。方法在锡耶纳大学医院信托基金会收集入组患者的咽拭子。采用qRT-PCR方法检测拭子中SARS-CoV-2及流感和人呼吸道合胞病毒（HRSV）等21种呼吸道病原体。通过下一代测序（NGS）进一步分析SARS-CoV-2和/或流感阳性拭子。结果从2024年1月至6月，入组了138例确诊为SARI的患者，平均年龄76.7岁，常见合并症。在这些患者中，40% %的人检测出SARS-CoV-2阳性，其次是20% %的人检测出流感阳性，13% %的人检测出HRSV阳性。对于SARS-CoV-2，发现了属于JN.1， EG.5和BA.5谱系的变体。甲型H1N1流感pdm09株属6B.1a.5a型。而对于甲型流感（H3N2），菌株属于两个不同的分支（3 C.2a1b.2a）。a和3 C.2a1b。2 a.2a.3a.1)。结论本研究有助于了解呼吸道病毒在急性呼吸道感染患者中的传播情况以及SARS-CoV-2和流感的遗传特征，强调持续监测的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Infection and Public Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH -INFECTIOUS DISEASES

CiteScore

13.10

自引率

1.50%

发文量

203

审稿时长

96 days

期刊介绍： The Journal of Infection and Public Health, first official journal of the Saudi Arabian Ministry of National Guard Health Affairs, King Saud Bin Abdulaziz University for Health Sciences and the Saudi Association for Public Health, aims to be the foremost scientific, peer-reviewed journal encompassing infection prevention and control, microbiology, infectious diseases, public health and the application of healthcare epidemiology to the evaluation of health outcomes. The point of view of the journal is that infection and public health are closely intertwined and that advances in one area will have positive consequences on the other. The journal will be useful to all health professionals who are partners in the management of patients with communicable diseases, keeping them up to date. The journal is proud to have an international and diverse editorial board that will assist and facilitate the publication of articles that reflect a global view on infection control and public health, as well as emphasizing our focus on supporting the needs of public health practitioners. It is our aim to improve healthcare by reducing risk of infection and related adverse outcomes by critical review, selection, and dissemination of new and relevant information in the field of infection control, public health and infectious diseases in all healthcare settings and the community.