Citrulline enhances salinity tolerance via photosynthesis, redox balance, osmotic and hormonal regulation, and nutrient assimilation in sunflower (Helianthus annuus L.).
Umer Farooq, Muhammad Arslan Ashraf, Rizwan Rasheed
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引用次数: 0
Abstract
Citrulline (CITR) is a strong osmolyte and hydroxyl radical scavenger. However, no previous study has reported the ameliorative role of CITR under salinity stress. We found a significant decrease in growth, chlorophyll content, SPAD value, photosynthesis, leaf relative water content, and nutrient acquisition in sunflower plants exposed to salinity (15 dS m‒1). Salinity caused substantial oxidative damage through elevating the levels of superoxide radicals (O2•‒), hydrogen peroxide (H2O2), hydroxyl radicals (·OH), leaf relative membrane permeability, malondialdehyde (MDA) and activity of lipoxygenase (LOX). Plants subjected to salinity manifested a higher buildup of methylglyoxal (MG), further exacerbating the cellular damage. However, CITR seed priming (1, 2, and 3 mM) partially relieved the negative repercussions of salinity by promoting the activities of antioxidant enzymes and levels of non-enzymatic antioxidants. Consequently, plants raised from CITR-primed seeds suffered less from oxidative damage and exhibited lower generation of O2·‒, H2O2, ·OH, MG, MDA, and activity of LOX. Plants under CITR supplementation exhibited higher chlorophyll content and improved efficiency of photosystem II as evidenced by higher values of maximum efficiency of photosystem-II (Fv/Fm), fraction of open PSII centers (qL), and photochemical quenching coefficient (qP). Citrulline priming enhanced plant resilience under salinity by improving hormonal balance, promoting polyamine accumulation, and sustaining photosynthetic performance. CITR bettered osmotic regulation through increased accumulation of osmolytes such as proline, glycine betaine, and total soluble sugars. Citrulline improved nutrient acquisition and diminished excess Na buildup, preventing specific ion toxicity and osmotic stress.
Supplementary information: The online version contains supplementary material available at 10.1007/s12298-025-01626-x.
期刊介绍：
Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.
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