[Methodological monitoring of the effects of endocrine disruptors in complex health maintenance].

Abstract

Introduction: By the 3rd millennium, the Earth as a living environment had been altered by environmental stresses. For 25 years, our research group has been thematically engaged in model studies of homeostatic disruptor effects. These have enabled thematic studies to follow the effects of chemical agents, e.g., the combined effects of chlorobenzenes (hexachlorobenzene : 1,2,4-trichlorobenzene 1 : 1), which have endocrine disrupting properties. Objective: We aimed to develop a test model system to investigate the effects of endocrine disruptors in a validated test system. Method: In the test system, male and female Wistar rats were used as model animals for in vivo studies. Subtoxicity was controlled by liver enzymes and body weight and physiological characterization. The exposure pathway was standardized. Then, the organism-level sub-toxic dose range and the time windows for acute and chronic monitoring of subtoxicity were defined. Plasma arginine vasopressin, and oxytocin levels were measured by RIA and LIA. Data were evaluated using SAS and ANOVA. Results: A stable in vivo control system had to be established to monitor the biological effects. The chlorobenzene exposures were standardized via gastrointestinal probe, in a final volume of 1.0 ml per day, at doses of 0.1 and 1.0 µg/kg body weight. The chlorobenzene potency model compounds were optimized during in vivo gastrointestinal exposure. Exposures were standardized: via gastrointestinal probe, 1 ml final volume per day, at doses of 0.1 and 1.0 µg/kg body weight. Experimental time intervals were 0–90 days. Discussion: In our work, we have succeeded in developing a test system to establish a validated method for monitoring chemical environmental exposures. We have also developed a standardised procedure for the determination of human toxicity potentials. Conclusion: A validated test system has been developed to test for persistent organic pollutants, which are nowadays a major health concern, making their testing of real relevance in clinical practice. Orv Hetil. 2025; 166(31): 1209–1216.