MicroRNA-31 mediated inhibition of keratin 6 by PSORI-CM01: a novel approach to psoriasis amelioration.

IF 4.2 3区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Frontiers in Chemistry Pub Date : 2025-07-18 eCollection Date: 2025-01-01 DOI:10.3389/fchem.2025.1636529

Shilei Wang, Jingkai Wang, Helin Pan, Ruogu Yang, Fanli Zeng, Yongfei Fang, Jinwei Zhang

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Abstract

Background: Psoriasis vulgaris is a serious noncommunicable disease, with no clear cause or cure. Expression of microRNA-31 (miR-31) is significantly increased in the cutaneous tissue of psoriasis vulgaris patients. Keratin 6 (Krt6) serves as a pivotal biomarker in the diagnostic and therapeutic approaches for psoriasis vulgaris. PSORI-CM01, a traditional Chinese medicine formulation comprising seven medicinal herbs, is employed in China for the therapeutic management of psoriasis vulgaris. However, its anti-psoriatic mechanism warrants further investigations. In this study, the underlying anti-psoriasis mechanism of PSORI-CM01dependent of miR-31 and Krt6 was explored.

Methods: In vivo, BALB/c mice were subjected to treatment with imiquimod (IMQ) to establish a psoriasis-like murine model. These psoriasis-like mice were then administered varying concentrations of PSORI-CM01. Following this, evaluations were performed on their Psoriasis Area and Severity Index (PASI) scores, epidermal thickness, and the expression levels of miR-31 and Krt6. HaCaT cells were subjected to treatment with interleukin-6 (IL-6) to create a psoriasis-like cellular model. Following this, the psoriasis-like keratinocytes were administered varying concentrations of PSORI-CM01, and the expression levels of miR-31 were quantified. In addition, these psoriasis-like keratinocytes were transfected with miR-31 mimics and subsequently treated with PSORI-CM01. The expression levels of Krt6 were then quantified and subjected to analysis.

Results: In vivo, PSORI-CM01 significantly alleviated the clinical-like manifestations of erythema, scales, and thickening in psoriasis-like mice, and it also reduced the PASI scores; Different concentrations of PSORI-CM01 significantly decreased epidermal thickness and the expression of miR-31 and Krt6 in psoriasis-like mice in a dose-dependent manner. In vitro, PSORI-CM01 significantly inhibited the expression of miR-31 and Krt6 in psoriasis-like keratinocytes; However, the decreased Krt6 protein expression was restored by miR-31 mimics.

Conclusion: PSORI-CM01 may improve psoriasis-like lesions by inhibiting expression of Krt6 protein dependent of miR-31.

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微rna -31介导的银屑病- cm01对角蛋白6的抑制：银屑病改善的新途径

背景：寻常型牛皮癣是一种严重的非传染性疾病，没有明确的病因和治疗方法。寻常型银屑病患者皮肤组织中microRNA-31 （miR-31）表达显著升高。角蛋白6 （Krt6）在寻常型银屑病的诊断和治疗方法中是一个关键的生物标志物。PSORI-CM01是一种由7种中草药组成的中药制剂，在中国用于治疗寻常型银屑病。然而，其抗银屑病机制有待进一步研究。本研究探讨了miR-31和Krt6依赖性的psori - cm01抗银屑病的潜在机制。方法：在体内用咪喹莫特（IMQ）治疗BALB/c小鼠，建立牛皮癣样小鼠模型。然后给这些牛皮癣样小鼠注射不同浓度的PSORI-CM01。随后，对他们的银屑病面积和严重程度指数（PASI）评分、表皮厚度以及miR-31和Krt6的表达水平进行评估。用白细胞介素-6 （IL-6）处理HaCaT细胞，建立牛皮癣样细胞模型。随后，对银屑病样角质形成细胞给予不同浓度的PSORI-CM01，并量化miR-31的表达水平。此外，用miR-31模拟物转染这些银屑病样角质形成细胞，随后用PSORI-CM01处理。然后定量分析Krt6的表达水平。结果：在体内，PSORI-CM01可显著缓解银屑病样小鼠的红斑、鳞片、增厚等临床样表现，降低PASI评分；不同浓度的银屑病- cm01以剂量依赖的方式显著降低银屑病样小鼠的表皮厚度和miR-31和Krt6的表达。在体外，银屑病- cm01显著抑制银屑病样角质形成细胞中miR-31和Krt6的表达；然而，miR-31模拟物恢复了Krt6蛋白表达的下降。结论：PSORI-CM01可能通过抑制miR-31依赖性Krt6蛋白的表达来改善银屑病样病变。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Chemistry Chemistry-General Chemistry

CiteScore

8.50

自引率

3.60%

发文量

1540

审稿时长

12 weeks

期刊介绍： Frontiers in Chemistry is a high visiblity and quality journal, publishing rigorously peer-reviewed research across the chemical sciences. Field Chief Editor Steve Suib at the University of Connecticut is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to academics, industry leaders and the public worldwide. Chemistry is a branch of science that is linked to all other main fields of research. The omnipresence of Chemistry is apparent in our everyday lives from the electronic devices that we all use to communicate, to foods we eat, to our health and well-being, to the different forms of energy that we use. While there are many subtopics and specialties of Chemistry, the fundamental link in all these areas is how atoms, ions, and molecules come together and come apart in what some have come to call the “dance of life”. All specialty sections of Frontiers in Chemistry are open-access with the goal of publishing outstanding research publications, review articles, commentaries, and ideas about various aspects of Chemistry. The past forms of publication often have specific subdisciplines, most commonly of analytical, inorganic, organic and physical chemistries, but these days those lines and boxes are quite blurry and the silos of those disciplines appear to be eroding. Chemistry is important to both fundamental and applied areas of research and manufacturing, and indeed the outlines of academic versus industrial research are also often artificial. Collaborative research across all specialty areas of Chemistry is highly encouraged and supported as we move forward. These are exciting times and the field of Chemistry is an important and significant contributor to our collective knowledge.