Synthesis Biomass Carbon Dots Derived From Carboxymethyl Chitosan and Epigallocatechin Gallate Both Antimicrobial and Antioxidation via a One-Step Hydrothermal Method

IF 3.4 4区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of biomedical materials research. Part B, Applied biomaterials Pub Date : 2025-08-04 DOI:10.1002/jbm.b.35630

Bingli Du, Jin Qi, Yanling Mi, Ran Zhang, Juan Ren, Yajuan Gong, Jiadi Li, Shuo Huang, Bing Li, Xiuping Wu

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Abstract

The resistance and biocompatibility of traditional antibiotics is a potential challenge for bacterial infections. Bacterial infections can produce oxidative stress, which can hamper cell migration and repair. In recent years, nanomaterials have become a viable alternative therapy for treating bacterial infections, with carbon dots (CDs) receiving popularity due to their superior characteristics. As a result, in this experiment, carboxymethyl chitosan (CMC) and epigallocatechin gallate (EGCG) were used as carbon sources, and multifunctional biomass green CDs with good biocompatibility, high antibacterial activity, potent antioxidant capacity, and the ability to promote cell migration and repair were synthesized by a one-step hydrothermal method. The cytotoxicity assay findings demonstrated that CDs had high biocompatibility and promoted cell growth at the concentration of 100 μg mL⁻¹. The results of the antibacterial experiment showed that CDs had a strong antibacterial effect upon Staphylococcus aureus and Escherichia coli. At the same time, CDs can eliminate ROS and protect cells from oxidative stress damage, which improves the cell's capacity to migrate and repair. The biomass green CDs provide a way to develop multifunctional carbon-based nanomaterials and provide a certain potential value for the promotion of wound healing and the clinical transformation of nanomaterials in the future.

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羧甲基壳聚糖和表没食子儿茶素没食子酸酯一步水热法制备抗菌抗氧化生物质碳点

传统抗生素的耐药性和生物相容性对细菌感染是一个潜在的挑战。细菌感染会产生氧化应激，阻碍细胞迁移和修复。近年来，纳米材料已成为治疗细菌感染的一种可行的替代疗法，碳点（cd）因其优越的特性而受到欢迎。因此，本实验以羧甲基壳聚糖（CMC）和表没食子儿茶素没食子酸酯（EGCG）为碳源，通过一步水热法合成了生物相容性好、抗菌活性高、抗氧化能力强、促进细胞迁移和修复能力强的多功能生物质绿色CDs。细胞毒性实验结果表明，在浓度为100 μg mL−1时，CDs具有较高的生物相容性，并能促进细胞生长。抑菌实验结果表明，CDs对金黄色葡萄球菌和大肠杆菌具有较强的抑菌作用。同时，cd可以消除ROS，保护细胞免受氧化应激损伤，提高细胞的迁移和修复能力。生物质绿色cd为开发多功能碳基纳米材料提供了一条途径，对未来促进伤口愈合和纳米材料的临床转化具有一定的潜在价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of biomedical materials research. Part B, Applied biomaterials 工程技术-材料科学：生物材料

CiteScore

7.50

自引率

2.90%

发文量

199

审稿时长

12 months

期刊介绍： Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats: • original research reports • short research and development reports • scientific reviews • current concepts articles • special reports • editorials Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.