Investigating the Antihyperplastic, Anti-Inflammatory and Antioxidant Properties of Cucurbita Pepo L. Seed Oil on Testosterone- Induced Benign Prostatic Hyperplasia (BPH) in Castrated Rat Model

IF 1.5 4区医学 Q3 UROLOGY & NEPHROLOGY

LUTS: Lower Urinary Tract Symptoms Pub Date : 2025-08-05 DOI:10.1111/luts.70024

Hajji Noura, Khémiri Ikram

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Abstract

Objective

The present study investigates the therapeutic potential of Cucurbita Pepo L. Seeds' Oil (CPSO) extracted by petroleum ether on testosterone-induced hyperplasia (BPH) in a castrated Wistar rat model.

Materials and Methods

Animals underwent two stages of treatment (28 days each): the testosterone treatment stage, to promote BPH, and the therapeutic stage, which consisted of an oral administration of CPSO. Forty-eight Wistar rats were equally divided into six groups: the negative control; groups 2–6 underwent a bilateral orchidectomy (induced via the scrotal route), then were redistributed into the castrated-control group and the castrated and testosterone-treated groups BPH induction (10 mg/kg). Twenty-eight days after BPH induction, the BPH rats were orally treated with Finasteride (FINA group: 5 mg/kg/d) and CPSO (CPSO-1 group: 40 mg/kg/d; CPSO-2 group: 80 mg/kg/d) for 28 additional days. Plasma biochemical parameters, circulating testosterone (T) and prostate-specific antigen (PSA), prostate oxidative markers, and inflammation were analyzed.

Results

CPSO administration exhibited a decrease in prostate weight, normalized the plasma total proteins, lipids, calcium and glucose, testosterone, and PSA levels, and attenuated the disturbances of liver and kidney functions in BPH rats. At the prostate level, CPSO showed a notably decreased prostate oxidation and inflammation. The histological study showed that CPSO restored prostatic lesions caused by BPH.

Conclusion

According to the present results, we suggest that CPSO could have a protective potential related to its proven anti-oxidant, anti-hyperplastic, and anti-inflammatory properties on preventing testosterone-induced BPH in castrated rats and might be used as an herbal remedy or nutraceutical agent in BPH management.

查看原文本刊更多论文

研究瓜籽油对睾丸激素诱导的良性前列腺增生（BPH）模型大鼠的抗增生性、抗炎和抗氧化作用

目的探讨石油醚提取葫芦籽油（CPSO）对阉割Wistar大鼠睾丸激素诱导增生症（BPH）的治疗作用。材料与方法动物分为两个治疗阶段（每个阶段28天）：睾酮治疗阶段，以促进BPH，治疗阶段，包括口服CPSO。48只Wistar大鼠平均分为6组：阴性对照组；2-6组行双侧睾丸切除术（经阴囊途径诱导），然后重新分为去势对照组和去势组和睾酮组BPH诱导（10 mg/kg）。BPH诱导28 d后，分别口服非那雄胺（FINA组：5 mg/kg/d）和CPSO (CPSO-1组：40 mg/kg/d)；CPSO-2组：80 mg/kg/d)，增加28 d。分析血浆生化指标、循环睾酮(T)、前列腺特异性抗原（PSA）、前列腺氧化标志物及炎症反应。结果CPSO能降低前列腺肥大大鼠的前列腺重量，使血浆总蛋白、血脂、钙、葡萄糖、睾酮和PSA水平正常化，减轻前列腺肥大大鼠肝肾功能紊乱。在前列腺水平，CPSO显示前列腺氧化和炎症明显减少。组织学研究表明，CPSO能恢复前列腺增生引起的前列腺病变。结论CPSO具有抗氧化、抗增生、抗炎等作用，可作为一种中药或营养品治疗去势大鼠BPH。

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来源期刊

LUTS: Lower Urinary Tract Symptoms UROLOGY & NEPHROLOGY-

CiteScore

3.00

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： LUTS is designed for the timely communication of peer-reviewed studies which provides new clinical and basic science information to physicians and researchers in the field of neurourology, urodynamics and urogynecology. Contributions are reviewed and selected by a group of distinguished referees from around the world, some of whom constitute the journal''s Editorial Board. The journal covers both basic and clinical research on lower urinary tract dysfunctions (LUTD), such as overactive bladder (OAB), detrusor underactivity, benign prostatic hyperplasia (BPH), bladder outlet obstruction (BOO), urinary incontinence, pelvic organ prolapse (POP), painful bladder syndrome (PBS), as well as on other relevant conditions. Case reports are published only if new findings are provided. LUTS is an official journal of the Japanese Continence Society, the Korean Continence Society, and the Taiwanese Continence Society. Submission of papers from all countries are welcome. LUTS has been accepted into Science Citation Index Expanded (SCIE) with a 2011 Impact Factor.