Analysis of the metagenomic assembled genome of MERS-CoV and Alpha-CoV from Camelus dromedarius in Saudi Arabia

IF 4 3区医学 Q1 INFECTIOUS DISEASES

Journal of Infection and Public Health Pub Date : 2025-07-20 DOI:10.1016/j.jiph.2025.102908

Ahmed M. Hassan , Muhammad Yasir , Tagreed Al-Subhi , Sayed Sartaj Sohrab , Aymn T. Abbas , Sherif A. El-Kafrawy , Taha Kumosani , Esam I. Azhar

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引用次数: 0

Abstract

Background

Dromedary camels are known carriers of Middle East respiratory syndrome coronavirus (MERS-CoV). This study aimed to identify respiratory RNA viruses circulating in the nasal cavities of camels that could potentially cause disease in humans.

Methods

Shotgun metagenomic paired-end sequencing was performed on four pools of nasal swabs collected from 40 camels originating from Sudan and Djibouti, using the MiSeq platform.

Results

Alphacoronavirus 229E was commonly detected in the camel pools. MERS-CoV was found in both pools from Sudan and one from Djibouti. Camel parainfluenza virus 3 (PIV3) was detected in the male camel pool from Sudan. Furthermore, near-complete (99.9 %) metagenomic assembled genomes (MAGs) of alphacoronavirus 229E (SIAU MAG01) and MERS-CoV (SIAU MAG02) were retrieved from the female camel pool from Sudan. Another MAG of alphacoronavirus 229E (SIAU MAG03) was recovered from a male camel pool imported from Sudan. In the phylogenetic analysis, SIAU MAG02 clustered with MERS-CoV genome sequences retrieved from humans and camels in the Middle East. The alphacoronavirus 229E MAGs from camels formed a distinct clade separate from the human alphacoronavirus 229E lineage. SIAU MAG04 clustered with PIV3 sequences recovered from Camelus dromedarius in the United Arab Emirates.

Conclusions

Overall, respiratory viruses belonging to alpha and beta coronaviruses, notably MERS-CoV, recognized by the World Health Organization as an emerging infectious disease of critical concern, were observed in dromedary camels from African origins, potentially posing a risk of transmission to humans.

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沙特阿拉伯骆驼骆驼MERS-CoV和α - cov元基因组组装基因组分析

背景：已知单峰骆驼是中东呼吸综合征冠状病毒（MERS-CoV）的携带者。这项研究的目的是识别在骆驼鼻腔中循环的呼吸道RNA病毒，这种病毒可能会导致人类患病。方法采用MiSeq平台对来自苏丹和吉布提的40头骆驼的4个鼻拭子进行shotgun元基因组配对端测序。结果在骆驼池中普遍检出229E型冠状病毒。在苏丹的两个池和吉布提的一个池中都发现了中东呼吸综合征冠状病毒。在苏丹雄性骆驼群中检测到骆驼副流感病毒3型（PIV3）。此外，从苏丹雌性骆驼池中检索到接近完整（99.9 %）的甲型冠状病毒229E （SIAU MAG01）和中东呼吸综合征冠状病毒（SIAU MAG02）宏基因组组装基因组（MAGs）。在从苏丹进口的雄性骆驼池中发现了另一种冠状病毒229E （SIAU MAG03）。在系统发育分析中，SIAU MAG02与中东地区人类和骆驼的MERS-CoV基因组序列聚类。来自骆驼的甲型冠状病毒229E mag形成了一个与人类甲型冠状病毒229E谱系分离的独特分支。SIAU MAG04与阿拉伯联合酋长国驼鹿（Camelus dromedarius）的PIV3序列聚类。结论总体而言，在来自非洲的单峰骆驼中观察到属于α和β冠状病毒的呼吸道病毒，特别是中东呼吸综合征冠状病毒，该病毒被世界卫生组织确认为一种严重关注的新兴传染病，可能存在传播给人类的风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Infection and Public Health PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH -INFECTIOUS DISEASES

CiteScore

13.10

自引率

1.50%

发文量

203

审稿时长

96 days

期刊介绍： The Journal of Infection and Public Health, first official journal of the Saudi Arabian Ministry of National Guard Health Affairs, King Saud Bin Abdulaziz University for Health Sciences and the Saudi Association for Public Health, aims to be the foremost scientific, peer-reviewed journal encompassing infection prevention and control, microbiology, infectious diseases, public health and the application of healthcare epidemiology to the evaluation of health outcomes. The point of view of the journal is that infection and public health are closely intertwined and that advances in one area will have positive consequences on the other. The journal will be useful to all health professionals who are partners in the management of patients with communicable diseases, keeping them up to date. The journal is proud to have an international and diverse editorial board that will assist and facilitate the publication of articles that reflect a global view on infection control and public health, as well as emphasizing our focus on supporting the needs of public health practitioners. It is our aim to improve healthcare by reducing risk of infection and related adverse outcomes by critical review, selection, and dissemination of new and relevant information in the field of infection control, public health and infectious diseases in all healthcare settings and the community.