Molecular marker assisted confirmation of a hybrid between Ascocentrum ampullaceum var. auranticum and Rhynchostylis retusa.

IF 1.6 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2025-08-05 DOI:10.1016/j.plgene.2025.100533

Kangabam Soneja Devi , Nandeibam Samarjit Singh , Heisnam Haripriyari Devi , Haobam Sharmila Devi , Huidrom Sunitibala Devi

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引用次数: 0

Abstract

Two rare and endangered orchids, Rhynchostylis retusa, and Ascocentrum ampullaceum var. auranticum, a narrowly endemic orchid from Manipur, were used for the present study. These two orchids were selected as parents because they have desirable traits like dense and floriferous, colorful, and long-lasting flowers. Our goal is to develop a hybrid species that is intermediate and improved over its parents in terms of floral characteristics by the F1 generation. When A. ampullaceum var. auranticum was the female parent, a 90 % crossability success rate was attained. Half-strength Murashige and Skoog (MS) basal medium with no phytohormones was used for seed germination in vitro and protocorm development of the putative hybrid. The basal medium containing 1 mg/L BAP and 0.5 mg/L NAA showed the highest growth response with 7.57 shoots/explant, 5.90 leaves/explant, and 57.19 PLBs/explant, while the medium enriched with 1 mg/L NAA produced the maximum number of roots (4.95) after 150 days of inoculation. The assessment for rapid determination of genetic purity of the developed hybrid (F1 plants) between A. ampullaceum var. auranticum and R. retusa was carried out by using the molecular markers, SCoT and SSR. The F1 plants' complementing banding patterns, which they inherited from their parents, confirmed that they were pure hybrids. These PCR-based molecular markers could be used for the early assessment of hybridity.

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分子标记辅助鉴定了壶形散囊草与黄纹花的杂种关系。

本研究以两种珍稀濒危兰花——蛇纹兰（Rhynchostylis retusa）和曼尼普尔（Manipur）特有的壶形兰（Ascocentrum ampullaceum var. aururanticum）为研究对象。这两种兰花之所以被选为亲本，是因为它们具有致密、多花、色彩鲜艳、开花时间长等令人满意的特点。我们的目标是开发一个杂交物种，是中间和改进其亲本方面的花卉特征的F1代。当壶叶金莲为母本时，杂交成功率达90%。用不含激素的半强度Murashige和Skoog （MS）基础培养基进行离体种子萌发和原球茎发育。BAP含量为1 mg/L、NAA含量为0.5 mg/L的基础培养基在接种150 d后生根数最多，达到7.57根/外植体、5.90叶/外植体和57.19根/外植体；NAA含量为1 mg/L的基础培养基在接种150 d后生根数最多，达到4.95根。利用分子标记、SCoT和SSR等方法，对ampullaceum var. aururanticum和r.r etusa杂交后代（F1植株）的遗传纯度进行了快速鉴定。这些F1植株的互补带状模式从它们的亲本遗传而来，证实了它们是纯杂交种。这些基于pcr的分子标记可用于杂交的早期评估。

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来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.