Combined desiccation treatments of hybrid larch (Larix x eurolepis) somatic embryos resulted in reduced water content keeping their germination and conversion rates: multi-scale characterizations

IF 6.2 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-12-01 Epub Date: 2025-07-23 DOI:10.1016/j.plaphy.2025.110283

Parisa Savane , Kateřina Eliášová , Petre Dobrev , Zuzana Vondráková , Armelle Delile , Nathalie Boizot , Odile Rogier , Václav Motyka , Céline Ridel , Stéphane Claverol , Caroline Teyssier , Marie-Anne Lelu-Walter

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引用次数: 0

Abstract

It is assumed that the production of somatic embryos (SEs), which closely resemble fully mature zygotic embryos (ZEs), increases the chance of producing somatic plants equivalent to seedlings. Desiccation is crucial to reduce the water content of SEs and to initiate biochemical changes that support germination and conversion into plants. We investigated the biological, histological, biochemical and molecular changes in the SEs of hybrid larch Larix eurolepis exposed to desiccation at high relative humidity (RH; 98 %), reduced RH (59 %), or a combination of both. Desiccation at 98 % RH (D98) resulted in starch reduction, sucrose synthesis and a decrease in abscisic acid content without affecting germination and plant frequencies. In addition, proteomic results showed that D98 directed SE metabolism towards the acquisition of desiccation tolerance and energy storage. These modifications were proved to be favorable for SE germination. Desiccation at 59 % RH (D59) reduced the water content to a level corresponding to that of dried zygotic embryos. It could negatively impact the germination rate. The response of D59 SEs was directed towards the activation of defense mechanisms, while modifications favoring desiccation tolerance were suppressed. The treatment, which combined the two desiccation modes successively, yielded the positive effects of D98 while reducing the water content of SEs, similar to D59. Overall, our results provide new insights into the processes associated with the desiccation of hybrid larch SEs. We have developed an effective desiccation method resulting in a reduced water content comparable to that of dry ZE, while ensuring a high germination rate and successful conversion into plants.

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杂交落叶松（Larix x eurolepis）体细胞胚联合干燥处理可降低其含水量，保持其萌发和转换率：多尺度特征

据推测，体细胞胚胎（SEs）的产生与完全成熟的合子胚胎（ZEs）非常相似，从而增加了产生相当于幼苗的体细胞植物的机会。干燥对于降低se的含水量和启动支持发芽和转化为植物的生化变化至关重要。研究了杂交落叶松（Larix eurolepis）在高相对湿度(RH；98%)， RH降低（59%），或两者兼有。98% RH （D98）下的干燥导致淀粉减少、蔗糖合成和脱落酸含量降低，但不影响发芽率和植株频率。此外，蛋白质组学结果表明，D98将SE代谢导向获得耐旱性和能量储存。结果表明，这些修饰有利于SE的萌发。在59% RH （D59）下的干燥使水分含量降低到与干燥的合子胚胎相当的水平。这可能会对发芽率产生负面影响。D59基因的响应指向防御机制的激活，而有利于干燥耐受的修饰被抑制。连续组合两种干燥方式的处理在降低se含水量的同时，对D98产生了积极的影响，与D59相似。总的来说，我们的研究结果为杂交落叶松SEs的干燥过程提供了新的见解。我们已经开发出一种有效的干燥方法，使含水量降低到与干燥ZE相当，同时确保高发芽率和成功转化为植物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.