Single-nucleotide polymorphisms (SNPs) in sperm adhesion molecule 1 (SPAM1) gene are associated with egg production in Yangzhou geese

IF 0.9 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-08-05 DOI:10.1016/j.genrep.2025.102311

Murtada Alsiddig , Tarig Badri , Hind Widaa , Bojiang Li , Honglin Liu

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引用次数: 0

Abstract

Sperm adhesion molecule 1(SPAM1) is a vital candidate gene that plays an important role in fertilization and hatchability of geese. In this study, we investigated for the first time the genetic variation of the SPAM1 gene and its association with egg production trait in Yangzhou geese. By using the direct sequencing technique, we detected three single nucleotide polymorphisms, g206 G>C, c123 T>A, and c159 T>C, located in the promoter and exon one regions, respectively. Six alleles and nine genotypes (GG, CC, GC, TT, TA, AA, TT, TC and CC) were obtained, respectively. The results indicated that the GG (g206 G>C) genotype had a significantly higher egg production rate during the 34-week egg-laying period. In the case of the SNPs c123 T > A and c159 T > C individuals with the AA and TT genotypes produced more eggs number, respectively. The SPAM 1 gene was highly expressed in the oviduct, abdominal fat, ovary and small intestine tissues. The mRNA expression level in the oviduct and ovary indicated that the geese with GG genotype recorded significantly lower expression levels (0.72 ± 0.02; 0.93 ± 0.02) compared to the geese with CC genotype (1.27 ± 0.19, 1.11 ± 0.06), respectively. Transcriptional activity results showed that both constructed vectors (pGL3-328G and pGL3-333C) had higher and more significant luciferase activity than the pGL3-basic vector. Future studies in Yangzhou and other native breeds of geese may be required to validate the association between these polymorphisms and egg production traits.

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扬州鹅精子粘附分子1 （SPAM1）基因的单核苷酸多态性与产蛋率有关

精子粘附分子1（Sperm adhesion molecule 1, SPAM1）是鹅受精和孵化过程中重要的候选基因。本研究首次研究了扬州鹅SPAM1基因的遗传变异及其与产蛋性状的关系。通过直接测序技术，我们检测到g206 G>；C、c123 T>；A和c159 T>；C三个单核苷酸多态性，分别位于启动子区和外显子1区。共获得6个等位基因和9个基因型（GG、CC、GC、TT、TA、AA、TT、TC和CC）。结果表明，GG （g206 G>；C）基因型在34周产蛋期产蛋率显著高于其他基因型。在snp c123 T >；A和c159 T >；AA和TT基因型的C个体分别产蛋量较多。SPAM - 1基因在输卵管、腹部脂肪、卵巢和小肠组织中高度表达。输卵管和卵巢mRNA表达量表明，GG基因型鹅的mRNA表达量显著低于对照组(0.72±0.02；0.93±0.02)，而CC基因型鹅分别为（1.27±0.19）、（1.11±0.06）。转录活性结果显示，构建载体pGL3-328G和pGL3-333C的荧光素酶活性均高于pGL3-basic载体。未来的研究可能需要在扬州和其他地方鹅品种中验证这些多态性与产蛋性状之间的关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.