Effect of eggshell calcium acetate on postmortem proteolysis and tenderization of spent duck muscle

IF 2 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Applied Poultry Research Pub Date : 2025-07-16 DOI:10.1016/j.japr.2025.100574

Ya-Shiou Chang

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引用次数: 0

Abstract

The purpose of this study was to investigate the effect of eggshell calcium acetate on postmortem proteolysis and tenderization of Tsaiya duck muscle. Duck carcasses were obtained from a government-regulated slaughterhouse and chilled at 12 °C for 1 h. Both sides of the breast muscles were excised and incubated individually in either eggshell calcium acetate solution containing 30 mM Ca²⁺ (ECA, N = 15), 30 mM calcium acetate (ACA, N = 15), or 30 mM EDTA (EDTA, N = 15) at 5 °C for 3 days. The control group (CON, N = 15) was incubated in double-distillated water at 5 °C for 3 days. Breast muscle samples from the right side of each carcass were taken at 0 (1 h postmortem), 1, 2, and 3 days of incubation for pH determination, casein zymography, and Western blotting. Samples from the left side of each breast were taken for myofibrillar fragmentation index (MFI) and shear force measurements at 1 and 3 days of incubation. The results showed that the decrease in calpain-1 and calpain-11 activities, and desmin content was more rapid in the ECA- and ACA-incubated samples compared to the CON and EDTA-incubated samples during the 3-day incubation period. On the other hand, the MFI increased significantly in the ECA- and ACA-incubated samples compared to the CON and EDTA-incubated samples, while shear force values decreased more in the ECA- and ACA-incubated samples than in the CON and EDTA-incubated samples. Our results suggest that eggshell calcium acetate, like calcium acetate, can effectively enhance postmortem proteolysis and tenderizing of spent duck breast muscles.

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蛋壳醋酸钙对鸭废肌死后蛋白水解及嫩化的影响

本试验旨在研究蛋壳醋酸钙对柴鸭死后蛋白水解和肉嫩化的影响。从政府监管的屠宰场获得鸭尸体，在12°C冷藏1小时。切除乳房两侧肌肉，分别在含有30 mM Ca2+ (ECA, N = 15)， 30 mM醋酸钙（ACA, N = 15）或30 mM EDTA （EDTA, N = 15）的蛋壳醋酸钙溶液中孵育，在5°C下孵育3天。对照组（CON, N = 15）在5℃双蒸馏水中孵育3 d。在孵育0（死后1小时）、1、2和3天分别从每具胴体右侧采集胸肌样本，进行pH测定、酪蛋白酶谱分析和Western blot检测。在孵育第1天和第3天分别从每个乳房左侧采集样本，测定肌纤维断裂指数（MFI）和剪切力。结果表明，与CON和edta孵育3 d的样品相比，ECA和aca孵育的样品中calpain-1和calpain-11活性以及desmin含量的下降速度更快。另一方面，与CON和edta孵育的样品相比，ECA和aca孵育的样品的MFI显著增加，而ECA和aca孵育的样品的剪切力值比CON和edta孵育的样品下降得更多。结果表明，蛋壳醋酸钙与醋酸钙一样，能有效促进鸭废胸肌的死后蛋白水解和嫩化。

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来源期刊

Journal of Applied Poultry Research 农林科学-奶制品与动物科学

CiteScore

4.10

自引率

10.50%

发文量

审稿时长

104 days

期刊介绍： The Journal of Applied Poultry Research (JAPR) publishes original research reports, field reports, and reviews on breeding, hatching, health and disease, layer management, meat bird processing and products, meat bird management, microbiology, food safety, nutrition, environment, sanitation, welfare, and economics. As of January 2020, JAPR will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. The readers of JAPR are in education, extension, industry, and government, including research, teaching, administration, veterinary medicine, management, production, quality assurance, product development, and technical services. Nutritionists, breeder flock supervisors, production managers, microbiologists, laboratory personnel, food safety and sanitation managers, poultry processing managers, feed manufacturers, and egg producers use JAPR to keep up with current applied poultry research.