Clinical evaluation of the ADVIA Centaur XPT chemiluminescent immunoassay for equine insulin measurement.

IF 1.6 3区 农林科学 Q2 VETERINARY SCIENCES
Journal of Equine Veterinary Science Pub Date : 2025-09-01 Epub Date: 2025-07-31 DOI:10.1016/j.jevs.2025.105661
R Rey-Conejo, R E Toribio, S Möller, E Müller, P Fores-Jackson
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引用次数: 0

Abstract

Background: Insulin dysregulation is a key component of equine metabolic syndrome and is commonly assessed using basal insulin concentrations. Different analyzers may yield variable insulin results, limiting comparability.

Aims/objectives: To assess the analytical performance of the ADVIA Centaur XPT chemiluminescent immunoassay (CLIA) for equine insulin measurement and to establish adapted decision thresholds based on comparison with a previously used CLIA method.

Methods: Precision, linearity, and dilution recovery were assessed for the ADVIA Centaur XPT. A total of 89 equine serum samples submitted for basal insulin testing were measured using the ADVIA Centaur XPT and Immulite 2000 XPi analyzers. Results were compared using Deming regression, Bland-Altman analysis, and weighted kappa statistics to evaluate agreement in classification.

Results: Intra-assay coefficients of variation were 3.56 %, 2.01 %, and 1.92 % for low, medium, and high insulin concentrations, respectively; inter-assay variation was 5.19 %, 5.78 %, and 5.68 %. Deming regression showed a proportional bias, with the ADVIA Centaur XPT consistently measuring lower insulin concentrations compared to the Immulite 2000 XPi. Bland-Altman analysis confirmed this bias across the measurement range. Spearman correlation between the two methods was 0.92 (95 % CI: 0.87-0.94; P < 0.0001), indicating a strong association in rank order. Classification agreement using adapted decision limits yielded a weighted kappa of 0.82, indicating strong agreement.

Conclusion: The ADVIA Centaur XPT demonstrated acceptable precision. When insulin concentrations were classified using adapted thresholds, the agreement between the two CLIA analyzers was strong, suggesting that the ADVIA Centaur XPT may be suitable for measurement of equine insulin concentration.

ADVIA Centaur XPT化学发光免疫分析法测定马胰岛素的临床评价。
背景:胰岛素失调是马代谢综合征的一个关键组成部分,通常用基础胰岛素浓度来评估。不同的分析仪可能产生不同的胰岛素结果,限制了可比性。目的/目的:评估ADVIA Centaur XPT化学发光免疫分析法(CLIA)用于马胰岛素测量的分析性能,并根据与先前使用的CLIA方法的比较建立适应的决策阈值。方法:对ADVIA Centaur XPT的精密度、线性度和稀释回收率进行评价。使用ADVIA Centaur XPT和Immulite 2000 XPi分析仪对提交的89份马血清样本进行基础胰岛素测试。比较结果采用Deming回归、Bland-Altman分析和加权kappa统计来评估分类的一致性。结果:低、中、高胰岛素浓度的测定内变异系数分别为3.56%、2.01%、1.92%;测定间变异率分别为5.19%、5.78%和5.68%。Deming回归显示出比例偏差,与Immulite 2000 XPi相比,ADVIA Centaur XPT持续测量较低的胰岛素浓度。Bland-Altman分析在测量范围内证实了这种偏差。两种方法的Spearman相关性为0.92 (95% CI: 0.87-0.94;P < 0.0001),表明在等级顺序上有很强的相关性。使用适应决策限制的分类一致产生0.82的加权kappa,表明高度一致。结论:ADVIA Centaur XPT具有可接受的精度。当使用适应阈值对胰岛素浓度进行分类时,两种CLIA分析仪之间的一致性很强,这表明ADVIA Centaur XPT可能适用于测量马胰岛素浓度。
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来源期刊
Journal of Equine Veterinary Science
Journal of Equine Veterinary Science 农林科学-兽医学
CiteScore
2.70
自引率
7.70%
发文量
249
审稿时长
77 days
期刊介绍: Journal of Equine Veterinary Science (JEVS) is an international publication designed for the practicing equine veterinarian, equine researcher, and other equine health care specialist. Published monthly, each issue of JEVS includes original research, reviews, case reports, short communications, and clinical techniques from leaders in the equine veterinary field, covering such topics as laminitis, reproduction, infectious disease, parasitology, behavior, podology, internal medicine, surgery and nutrition.
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