A practical and easy-to-scale protocol for removing chlorophylls from leaf extracts

IF 2.4 3区生物学 Q2 PLANT SCIENCES

Applications in Plant Sciences Pub Date : 2025-07-29 DOI:10.1002/aps3.70018

Alice Fossati, Valeria Cavalloro, Daniela Rossi, Simona Collina, Emanuela Martino

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Abstract

Premise

Leaf extracts are valuable sources of bioactive compounds. However, co-extracted chlorophylls interfere with analyses, including spectroscopic and biochemical assays. Existing methods for chlorophyll removal often have limitations, including the use of hazardous solvents, low specificity, or high costs.

Methods and Results

A solid-phase extraction protocol for chlorophyll removal from leaf extracts of Corylus avellana was developed using commercially available cartridges. The method requires standard equipment, can be completed within 10 minutes, and is scalable from analytical to preparative quantities. We validated this protocol across 20 taxa, demonstrating the removal of 85% of chlorophylls, successful scale up of quantities, cartridge reusability, and low solvent consumption.

Conclusions

Key innovations of the protocol include simplified elution steps and the possibility of multiple reuse cycles. The simplicity, sustainability, and scalability of this new protocol make it particularly valuable for high-throughput applications and process development.

Abstract Image

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从叶片提取物中去除叶绿素的一种实用且易于扩展的方案

前提是叶提取物是生物活性化合物的宝贵来源。然而，共提取叶绿素干扰分析，包括光谱和生化分析。现有的叶绿素去除方法通常有局限性，包括使用有害溶剂、特异性低或成本高。方法与结果建立了一种利用市售胶筒从榛叶提取物中提取叶绿素的固相萃取方法。该方法需要标准设备，可在10分钟内完成，并且可从分析量扩展到制备量。我们在20个分类群中验证了该方案，证明了85%的叶绿素被去除，数量成功扩大，墨盒可重复使用，溶剂消耗低。结论该方案的关键创新在于简化了洗脱步骤和多次重复使用的可能性。这种新协议的简单性、可持续性和可伸缩性使其对高吞吐量应用程序和流程开发特别有价值。

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来源期刊

Applications in Plant Sciences PLANT SCIENCES-

CiteScore

7.30

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Applications in Plant Sciences (APPS) is a monthly, peer-reviewed, open access journal promoting the rapid dissemination of newly developed, innovative tools and protocols in all areas of the plant sciences, including genetics, structure, function, development, evolution, systematics, and ecology. Given the rapid progress today in technology and its application in the plant sciences, the goal of APPS is to foster communication within the plant science community to advance scientific research. APPS is a publication of the Botanical Society of America, originating in 2009 as the American Journal of Botany''s online-only section, AJB Primer Notes & Protocols in the Plant Sciences. APPS publishes the following types of articles: (1) Protocol Notes describe new methods and technological advancements; (2) Genomic Resources Articles characterize the development and demonstrate the usefulness of newly developed genomic resources, including transcriptomes; (3) Software Notes detail new software applications; (4) Application Articles illustrate the application of a new protocol, method, or software application within the context of a larger study; (5) Review Articles evaluate available techniques, methods, or protocols; (6) Primer Notes report novel genetic markers with evidence of wide applicability.