Drought-Induced genomic and epigenetic variations in Quinoa genotypes revealed by iPBS and CRED-iPBS marker systems.

IF 3.9 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Scientific Reports Pub Date : 2025-08-01 DOI:10.1038/s41598-025-13370-z

Aras Türkoğlu, Kamil Haliloğlu, Fatih Demirel, Serap Demirel, Muhammet İslam Işık, Adnan Aydin, Mustafa Tan, Hadi Alipour

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Abstract

Drought stress significantly impacts crop productivity, yet its influence on genomic and epigenetic variation in quinoa remains poorly understood. This study aimed to assess DNA damage and cytosine methylation alterations in six quinoa genotypes (Titicaca, Rainbow, Moqu Arrochilla, Cherry Vanilla, China, and White) exposed to five irrigation levels (5%, 10%, 25%, 50%, and 100% field capacity). Genomic changes were evaluated using inter-primer binding site (iPBS) markers, while DNA methylation was analyzed via CRED-iPBS. Results revealed genotype-specific polymorphism and genomic template stability (GTS) responses to irrigation stress. Moqu Arrochilla showed the highest GTS (84.6%) under 5% field capacity, while White exhibited the lowest (35.0%) at 50% field capacity. CRED-iPBS analysis indicated both hyper- and hypomethylation events depending on stress intensity, with China and Moqu Arrochilla genotypes displaying the highest polymorphism rates for MspI (42.9%) and HpaII (39.0%), respectively. These findings highlight the drought-induced genomic and epigenetic variability in quinoa, emphasizing the utility of iPBS and CRED-iPBS techniques for screening stress-responsive genotypes. This work contributes to the understanding of stress adaptation and may inform breeding programs targeting drought tolerance.

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iPBS和CRED-iPBS标记系统揭示的干旱诱导的藜麦基因型基因组和表观遗传变异

干旱胁迫显著影响作物生产力，但其对藜麦基因组和表观遗传变异的影响尚不清楚。本研究旨在评估6种藜麦基因型（Titicaca、Rainbow、Moqu Arrochilla、Cherry Vanilla、China和White）暴露在5种灌溉水平（5%、10%、25%、50%和100%田间容量）下的DNA损伤和胞嘧啶甲基化变化。使用引物结合位点（iPBS）标记评估基因组变化，通过CRED-iPBS分析DNA甲基化。结果揭示了基因型特异性多态性和基因组模板稳定性（GTS）对灌溉胁迫的响应。Moqu Arrochilla在5%的田间容量下GTS最高（84.6%），而White在50%的田间容量下GTS最低（35.0%）。CRED-iPBS分析显示，高甲基化和低甲基化事件取决于胁迫强度，中国和Moqu基因型的MspI和HpaII多态性率分别最高（42.9%）和39.0%)。这些发现强调了干旱诱导的藜麦基因组和表观遗传变异，强调了iPBS和CRED-iPBS技术在筛选应激反应基因型方面的应用。这项工作有助于了解胁迫适应，并可能为针对耐旱性的育种计划提供信息。

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来源期刊

Scientific Reports Natural Science Disciplines-

CiteScore

7.50

自引率

4.30%

发文量

19567

审稿时长

3.9 months

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