miRNA-29c-3p Activates the JAK2/STAT3 Signaling Pathway by Down-Regulating SOCS3 to Promote Pathological Angiogenesis and Inflammation in Diabetic Retinopathy.

IF 1 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY

Annals of clinical and laboratory science Pub Date : 2025-05-01

Xiao-Mei Chen, Min Wen, Rong Wu, Jin-Feng Xie, Nian-Lian Wen, Ting-Hui Fan, Sheng Huang

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引用次数: 0

Abstract

Objective: The purpose of this study was to explore the effects and potential mechanisms of miRNA-29c-3p regulating suppressors of cytokine signaling 3 (SOCS3) in Diabetic Retinopathy (DR) progression.

Methods: Human retinal microvascular endothelial cells (hRMECs) were exposed to 25 mM high glucose concentrations to establish a DR cell model and underwent transfection to down-regulate miRNA-29c-3p and SOCS3.

Results: High glucose induces upregulation of miRNA-29c-3p and downregulation of SOCS3 expression in hRMECs. Under high-glucose conditions, inhibition of miRNA-29c-3p significantly suppresses hRMEC migration, angiogenesis, and the release of pro-inflammatory cytokines. Notably, this inhibitory effect is partially reversed upon SOCS3 knockdown. Moreover, miRNA-29c-3p directly targets and regulates SOCS3 mRNA expression. Importantly, SOCS3 knockdown markedly activates the JAK2/STAT3 signaling pathway in hRMECs, which can be suppressed by reducing miRNA-29c-3p levels.

Conclusion: miRNA-29c-3p promotes DR progression by activating the JAK2/STAT3 pathway through SOCS3 regulation.

本刊更多论文

miRNA-29c-3p通过下调SOCS3激活JAK2/STAT3信号通路促进糖尿病视网膜病变病理性血管生成和炎症

目的：探讨miRNA-29c-3p调节细胞因子信号传导3抑制因子（SOCS3）在糖尿病视网膜病变（DR）进展中的作用及其可能机制。方法：将人视网膜微血管内皮细胞（hRMECs）暴露于25 mM高葡萄糖环境中，建立DR细胞模型，转染下调miRNA-29c-3p和SOCS3。结果：高糖诱导hrmec中miRNA-29c-3p表达上调，SOCS3表达下调。在高糖条件下，抑制miRNA-29c-3p可显著抑制hRMEC迁移、血管生成和促炎细胞因子的释放。值得注意的是，这种抑制作用在SOCS3敲除后部分逆转。此外，miRNA-29c-3p直接靶向并调控SOCS3 mRNA的表达。重要的是，SOCS3敲低显著激活hRMECs中的JAK2/STAT3信号通路，这可以通过降低miRNA-29c-3p水平来抑制。结论：miRNA-29c-3p通过SOCS3调控激活JAK2/STAT3通路，促进DR进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of clinical and laboratory science 医学-医学实验技术

CiteScore

1.60

自引率

0.00%

发文量

112

审稿时长

6-12 weeks

期刊介绍： The Annals of Clinical & Laboratory Science welcomes manuscripts that report research in clinical science, including pathology, clinical chemistry, biotechnology, molecular biology, cytogenetics, microbiology, immunology, hematology, transfusion medicine, organ and tissue transplantation, therapeutics, toxicology, and clinical informatics.