Identification of novel Listeria monocytogenes serotype 4h with specific antisera against wall teichoic acid

IF 1.9 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2025-08-03 DOI:10.1016/j.mimet.2025.107210

Zhenhua Wu , Hao Yao , Jing Li , Fanzeng Meng , Chao Chen , Ye Wang , Yuelan Yin , Xin'an Jiao

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引用次数: 0

Abstract

Listeria monocytogenes (Lm) strains are serotyped based on their somatic (O) and flagellar (H) antigens. The newly designated serotype (ST) 4h exhibits a greater ability to colonize organs than well-characterized hypervirulent strains. Therefore, simple and rapid identification methods for Lm ST 4h are urgently needed for effective monitoring and prevention. Here, we report the development of rabbit polyclonal antibodies against the O-antigen of Lm ST 4h, targeting its unique galactosylated wall teichoic acid (WTA) structure. These antisera exhibit high titers and enable the rapid and accurate identification of ST 4h isolates via slide agglutination and ELISA assays, demonstrating their specificity in labeling Lm ST 4h. The WTA-antisera provide a valuable tool for investigating the pathogenic mechanisms of hypervirulent strains and enhancing existing methods for Lm ST identification.

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新型单核增生李斯特菌血清型4h抗壁壁酸特异性血清的鉴定

单核增生李斯特菌（Lm）菌株根据其体细胞(O)和鞭毛(H)抗原进行血清分型。新指定的血清型（ST） 4h比特性良好的高毒菌株在器官上定植的能力更强。因此，迫切需要简便快速的Lm ST 4h鉴定方法，以便进行有效的监测和预防。在这里，我们报道了针对Lm ST 4h o抗原的兔多克隆抗体的开发，靶向其独特的半乳糖基化壁壁壁酸（WTA）结构。这些抗血清具有高滴度，能够通过玻片凝集和ELISA检测快速准确地鉴定ST 4h分离物，证明它们在标记Lm ST 4h方面具有特异性。wta抗血清为研究高毒菌株的致病机制和改进现有的Lm ST鉴定方法提供了有价值的工具。

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.