Stage-specific and cumulative effects of heat stress on male and female reproductive performance in Drosophila melanogaster

IF 2.9 2区 生物学 Q2 BIOLOGY
Abhishek Meena, Komal Maggu, Alessio N. De Nardo, Viktor Kovalov, Benjamin Eggs, Stefan Lüpold
{"title":"Stage-specific and cumulative effects of heat stress on male and female reproductive performance in Drosophila melanogaster","authors":"Abhishek Meena,&nbsp;Komal Maggu,&nbsp;Alessio N. De Nardo,&nbsp;Viktor Kovalov,&nbsp;Benjamin Eggs,&nbsp;Stefan Lüpold","doi":"10.1016/j.jtherbio.2025.104213","DOIUrl":null,"url":null,"abstract":"<div><div>Frequent heat events, driven by climate change, pose significant threats to biodiversity, particularly for insects and other ectothermic species with limited thermoregulatory capacities. Beyond affecting survival, heat stress can severely impair reproductive fitness. Previous studies have typically examined the effects of heat events on single reproductive stages or sexes. However, the thermal sensitivity likely varies across sex-specific reproductive stages, from gonad development to post-mating gamete interactions. Here, we investigated the timing- and sex-specific effects of heat stress across key reproductive stages in <em>Drosophila melanogaster.</em> Using a full-factorial design, we exposed males and females to heat stress during development, as premating adults, and after mating to assess stage-specific and cumulative impacts on reproductive performance. Our results revealed the highest thermal sensitivity during post-mating sperm storage by females, where even brief heat stress critically reduced reproductive output. Pre-mating adult heat exposure also affected both sexes, but less severely, whilst developmental heat exposure had the least impact and mostly on males. These findings highlight the critical role of timing and sex-specific reproductive stages in mediating thermal fertility. Our study provides novel insights into the vulnerability of sex-specific reproductive stages to heat stress, offering essential knowledge for predicting more accurate population dynamics and persistence under increasingly variable thermal conditions driven by climate change.</div></div>","PeriodicalId":17428,"journal":{"name":"Journal of thermal biology","volume":"132 ","pages":"Article 104213"},"PeriodicalIF":2.9000,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of thermal biology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0306456525001706","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Frequent heat events, driven by climate change, pose significant threats to biodiversity, particularly for insects and other ectothermic species with limited thermoregulatory capacities. Beyond affecting survival, heat stress can severely impair reproductive fitness. Previous studies have typically examined the effects of heat events on single reproductive stages or sexes. However, the thermal sensitivity likely varies across sex-specific reproductive stages, from gonad development to post-mating gamete interactions. Here, we investigated the timing- and sex-specific effects of heat stress across key reproductive stages in Drosophila melanogaster. Using a full-factorial design, we exposed males and females to heat stress during development, as premating adults, and after mating to assess stage-specific and cumulative impacts on reproductive performance. Our results revealed the highest thermal sensitivity during post-mating sperm storage by females, where even brief heat stress critically reduced reproductive output. Pre-mating adult heat exposure also affected both sexes, but less severely, whilst developmental heat exposure had the least impact and mostly on males. These findings highlight the critical role of timing and sex-specific reproductive stages in mediating thermal fertility. Our study provides novel insights into the vulnerability of sex-specific reproductive stages to heat stress, offering essential knowledge for predicting more accurate population dynamics and persistence under increasingly variable thermal conditions driven by climate change.
热应激对黑腹果蝇雄性和雌性生殖性能的阶段特异性和累积效应
由气候变化驱动的频繁高温事件对生物多样性构成重大威胁,特别是对温度调节能力有限的昆虫和其他恒温物种。除了影响生存,热应激还会严重损害生殖健康。以前的研究主要是研究炎热事件对单个生殖阶段或性别的影响。然而,从性腺发育到交配后配子相互作用,热敏感性可能在性别特定的生殖阶段有所不同。在这里,我们研究了热应激在黑腹果蝇关键生殖阶段的时间和性别特异性影响。使用全因子设计,我们将雄性和雌性在发育期间、作为早熟成虫和交配后暴露于热应激中,以评估对生殖性能的特定阶段和累积影响。我们的研究结果显示,雌性在交配后的精子储存过程中,温度敏感性最高,即使短暂的热应激也会严重降低生殖产量。交配前的成年热暴露对两性也有影响,但不太严重,而发育期的热暴露对雄性的影响最小。这些发现强调了时间和性别特异性生殖阶段在调节热生育中的关键作用。我们的研究为性别特异性生殖阶段对热应激的脆弱性提供了新的见解,为在气候变化驱动的日益变化的热条件下更准确地预测种群动态和持久性提供了必要的知识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of thermal biology
Journal of thermal biology 生物-动物学
CiteScore
5.30
自引率
7.40%
发文量
196
审稿时长
14.5 weeks
期刊介绍: The Journal of Thermal Biology publishes articles that advance our knowledge on the ways and mechanisms through which temperature affects man and animals. This includes studies of their responses to these effects and on the ecological consequences. Directly relevant to this theme are: • The mechanisms of thermal limitation, heat and cold injury, and the resistance of organisms to extremes of temperature • The mechanisms involved in acclimation, acclimatization and evolutionary adaptation to temperature • Mechanisms underlying the patterns of hibernation, torpor, dormancy, aestivation and diapause • Effects of temperature on reproduction and development, growth, ageing and life-span • Studies on modelling heat transfer between organisms and their environment • The contributions of temperature to effects of climate change on animal species and man • Studies of conservation biology and physiology related to temperature • Behavioural and physiological regulation of body temperature including its pathophysiology and fever • Medical applications of hypo- and hyperthermia Article types: • Original articles • Review articles
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信