Chemical Profile and Biological Activity of the Hymenochaetoid Mushroom Hirschioporus fuscoviolaceus (Agaricomycetes).

IF 1.4
Suratno Suratno, Bianka Várnai, Csenge Anna Felegyi-Tóth, Viktor Papp, Imre Boldizsár, Tamás Gáti, Ágnes M Móricz, Szabolcs Béni, Attila Vanyolos
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Abstract

Hirschioporus fuscoviolaceus is a commonly distributed saprotrophic fungus across the coniferous forests of temperate regions. An in-depth chemical analysis of the methanol extract of H. fuscoviolaceus resulted in the isolation of four compounds (1-4). The four fungal metabolites were identified as ergosterol peroxide (1), 5α,8α-epidioxy-ergosta-6-en-3β-ol (2), β-sitosterol (3) and 9,11-dehydro-ergosterol peroxide (4), that latest was isolated as a mixture with its closely related compound, ergosterol peroxide (1). The structure identification of the isolated compounds was carried out by one- and two-dimensional NMR and MS analysis. The antimicrobial properties of the fungal secondary metabolites were investigated on several pathogens including Bacillus subtilis, Rhodococcus fascians, Xanthomonas euvesicatoria, and Fusarium graminearum. According to our results, among the identified ergostane-type sterols only mixture of compounds 1 and 4 exhibited moderate inhibitory activity on Bacillus subtilis and Rhodococcus fascians. For the examination of their potential α-glucosidase, lipase and acetylcholinesterase inhibition activities, dot-blot enzyme assays were performed which highlighted that compounds 1-4 have considerable α-glucosidase inhibitory property, with the most active isolates of 3 and 4, while compounds 1, 2 and 4 demonstrated notable activity against acetylcholinesterase. The current study represents the first report on the chemical profile of H. fuscoviolaceus, providing a comprehensive study on the isolation and structure elucidation of the most important secondary metabolites and their potential biological activities.

漆膜类蘑菇fuscoviolaceus的化学特征和生物活性研究。
褐紫Hirschioporus fuscoviolaceus是一种普遍分布于温带针叶林的腐养真菌。对fuscoviolaceus的甲醇提取物进行了深入的化学分析,分离出四种化合物(1-4)。鉴定出4种真菌代谢产物分别为麦角甾醇过氧化物(1)、5α,8α-附二氧基麦角-6-烯-3β-醇(2)、β-谷甾醇(3)和9,11-脱氢麦角甾醇过氧化物(4),最后分离得到与其密切相关的化合物麦角甾醇过氧化物(1)的混合物。通过核磁共振和质谱对分离得到的化合物进行了结构鉴定。研究了该真菌次生代谢物对枯草芽孢杆菌、fasdococcus fascian、euvesicatoria黄单胞菌和Fusarium graminearum等病原菌的抑菌性能。根据我们的研究结果,在鉴定的麦角烷型甾醇中,只有化合物1和4的混合物对枯草芽孢杆菌和筋膜红球菌具有中等的抑制活性。对化合物1 ~ 4的α-葡萄糖苷酶、脂肪酶和乙酰胆碱酯酶的抑制活性进行了点印迹酶检测,结果表明化合物3和4对α-葡萄糖苷酶的抑制活性最高,而化合物1、2和4对乙酰胆碱酯酶的抑制活性最高。本研究首次报道了fuscoviolaceus的化学特征,对其最重要的次生代谢产物的分离、结构解析及其潜在的生物活性进行了全面的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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