Microbial contamination of air filters of air conditioning system of urban buses.

IF 0.5 4区 医学 Q4 MICROBIOLOGY
D Obitková, C Čereiová, M Mráz, E Pavlík
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Abstract

The use of HVAC in urban buses in developed countries increases the comfort and indoor air quality in the means of ground transportation. The microbial contamination was studied on outlet and inlet surfaces of 5 air filters removed from the urban buses HVAC during regular maintenance. To acquire samples from both the outlet and the inlet sides of the filters, dry swabbing technique was used. Cultivation was performed on different selective or selective-diagnostic agars, to cultivate viable bacteria. To identify the bacterial species, Gram stain and immerse microscopy was used. Selected colonies underwent the proteomic study (MALDI-TOF) as well. After identification, bacteria were quantified. The bacteria of the genus Bacillus - Bacillus cereus, Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Bacillus flexus prevailed on both inlet and outlet surfaces of the filters. The members of genera Staphylococcus, Brevibacillus, Peribacillus or Paenibacillus were also identified. The quantification of colony forming units showed low contamination of the outlet surfaces of filters 1 and 2. The contamination of inlet and outlet sides of filters 3, 4, and 5 was comparable, revealed nearly the same contamination of inlet and outlet surfaces. In the case of filters 3, 4 and 5 we recommend more frequent filter changing or more efficient filter choice.

城市客车空调系统空气过滤器微生物污染。
发达国家在城市公交车上使用暖通空调,提高了地面交通工具的舒适性和室内空气质量。对定期检修时从城市公交车暖通空调上拆下的5个空气过滤器的出风口表面和进风口表面进行了微生物污染研究。为了从过滤器的出口和进口两侧获取样本,使用了干拭子技术。在不同的选择性或选择性诊断琼脂上进行培养,培养活菌。采用革兰氏染色法和浸泡镜检法鉴定细菌种类。选择的菌落也进行了蛋白质组学研究(MALDI-TOF)。鉴定后对细菌进行定量。芽孢杆菌属细菌-蜡样芽孢杆菌、枯草芽孢杆菌、地衣芽孢杆菌、细芽孢杆菌、弯曲芽孢杆菌在过滤器的入口和出口表面均占主导地位。还鉴定了葡萄球菌属、短芽孢杆菌属、芽孢杆菌属和芽孢杆菌属的成员。菌落形成单元的定量分析表明,1号和2号滤池的出口表面污染程度较低。过滤器3、4和5的进口和出口两侧的污染具有可比性,表明进口和出口表面的污染几乎相同。在过滤器3、4和5的情况下,我们建议更频繁地更换过滤器或选择更有效的过滤器。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Epidemiologie Mikrobiologie Imunologie
Epidemiologie Mikrobiologie Imunologie Medicine-Immunology and Allergy
CiteScore
0.90
自引率
0.00%
发文量
20
期刊介绍: The journal publishes original papers, information from practice, reviews on epidemiological and microbiological subjects. Sufficient space is devoted to diagnostic methods from medical microbiology, parasitology, immunology, and to general aspects and discussions pertaining to preventive medicine. It also brings translations and book reviews useful for medical doctors and research workers and professionals in public health.
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