Optimising Filtered Egg Yolk Extenders for Long-Term Cold Preservation of Ram Sperm: Effects of Osmolarity and Egg Yolk Concentration.

IF 1.7 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Reproduction in Domestic Animals Pub Date : 2025-08-01 DOI:10.1111/rda.70106

Mohammad Hassan Nategh Ahmadi, Abdolah Mirzaei, Mohammad Reza Divar, Fatemeh Derafshani

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Abstract

Cold storage is a preferred method for short-term preservation of ram spermatozoa due to its superior fertility rates compared to cryopreservation. This study aimed to optimise the conditions for ram sperm preservation by evaluating the effects of osmolarity and egg yolk (EY) concentration in a syringe-filtered extender. In part A, semen samples were extended with solutions of varying osmolarities (330, 360, 390 and 420 mOsm/kg water). The 390 mOsm/kg solution demonstrated the best preservation of sperm viability, functional integrity and motility over 96 h of storage. In part B, different EY concentrations (10%, 15%, 20%, 25% and 30%) were assessed at the optimal osmolarity (390 mOsm/kg). A 20% EY concentration provided the most effective protection of sperm DNA, acrosome integrity and membrane functionality. Excessive EY levels (> 20%) negatively impacted sperm viability and induced higher lipid peroxidation. These findings emphasise the importance of balancing osmolarity and EY concentration for efficient cold storage of ram spermatozoa, enhancing the potential success of artificial insemination programs in sheep breeding.

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用于长期低温保存公羊精子的过滤蛋黄填充剂的优化：渗透压和蛋黄浓度的影响。

冷库是短期保存公羊精子的首选方法，因为与冷冻保存相比，冷库的生育能力更强。本研究旨在通过评估渗透浓度和蛋黄（EY）浓度对注射器过滤膨化剂中公羊精子保存的影响来优化条件。在A部分，精液样品用不同渗透压的溶液（330、360、390和420 mOsm/kg水）进行扩展。390 mOsm/kg的溶液在96 h的保存时间内，精子的活力、功能完整性和活力得到了最好的保存。在B部分，在最佳渗透压（390 mOsm/kg）下，评估不同的EY浓度（10%、15%、20%、25%和30%）。20%的EY浓度对精子DNA、顶体完整性和膜功能提供最有效的保护。过高的EY水平（bbb20 %）会对精子活力产生负面影响，并引起更高的脂质过氧化。这些发现强调了平衡渗透压和EY浓度对于有效冷藏公羊精子的重要性，提高了绵羊育种中人工授精计划的潜在成功率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproduction in Domestic Animals 农林科学-奶制品与动物科学

CiteScore

3.00

自引率

5.90%

发文量

238

审稿时长

4-8 weeks

期刊介绍： The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.