TcJAMYC5 positively regulates paclitaxel biosynthesis in Taxus chinensis var. Mairei.

IF 3.8 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Molecular Biology Pub Date : 2025-07-31 DOI:10.1007/s11103-025-01626-1

Shiyu Cai, Lina Xue, Ziling Tao, Fengxiu Li, Qiao Liu, Wen Wan, Jihong Jiang, Ludan Li, Xiaoying Cao

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引用次数: 0

Abstract

Paclitaxel is an important natural anticancer drug. Its biosynthesis is very complex and 18 enzymes likely involved have been characterized. However, the regulatory mechanism of these enzyme genes still remains to be elucidated. Here we identified a novel transcription factor in the MYC family of Taxus chinensis TcJAMYC5 function in paclitaxel biosynthesis. TcJAMYC5 was highly expressed in the roots and regulated by MeJA. Transient overexpression of TcJAMYC5 in T. chinensis cambial meristematic cells resulted in a significant increase in paclitaxel and bacctin III content and upregulated expression of nearly all of paclitaxel biosynthesis related genes except T13αOH. Suppression of TcJAMYC5 expression in cambial meristematic cells resulted in a significant decrease in paclitaxel content. TcJAMYC5 could bind to promoters of paclitaxel biosynthesis pathway enzyme genes TASY, DBTNBT and T5αH for directly activating their expression. Taken together, we conclude that TcJAMYC5 is an activator that improves the accumulation of paclitaxel in T. chinensis through a MeJA-medicated signaling pathway.

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TcJAMYC5正调控中国红豆杉（Taxus chinensis var. marei）紫杉醇合成。

紫杉醇是一种重要的天然抗癌药物。其生物合成过程非常复杂，目前已知可能涉及的酶有18种。然而，这些酶基因的调控机制仍有待阐明。本研究鉴定了红豆杉MYC家族中一个新的转录因子TcJAMYC5在紫杉醇生物合成中的作用。TcJAMYC5在根中高表达，受MeJA调控。TcJAMYC5在中国冬青形成层分生组织细胞中短暂过表达，导致紫杉醇和bacctin III含量显著增加，除T13αOH外，几乎所有紫杉醇生物合成相关基因的表达上调。抑制形成层分生组织细胞中TcJAMYC5的表达导致紫杉醇含量显著降低。TcJAMYC5可以结合紫杉醇生物合成通路酶基因TASY、DBTNBT和T5αH的启动子，直接激活它们的表达。综上所述，我们认为TcJAMYC5是通过meja介导的信号通路促进紫杉醇积累的激活因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Molecular Biology 生物-生化与分子生物学

自引率

2.00%

发文量

审稿时长

1.4 months

期刊介绍： Plant Molecular Biology is an international journal dedicated to rapid publication of original research articles in all areas of plant biology.The Editorial Board welcomes full-length manuscripts that address important biological problems of broad interest, including research in comparative genomics, functional genomics, proteomics, bioinformatics, computational biology, biochemical and regulatory networks, and biotechnology. Because space in the journal is limited, however, preference is given to publication of results that provide significant new insights into biological problems and that advance the understanding of structure, function, mechanisms, or regulation. Authors must ensure that results are of high quality and that manuscripts are written for a broad plant science audience.