Mitochondrial genome and phylogeny of American silk moth Antheraea polyphemus and its Asian relatives (Lepidoptera: Saturniidae).

Abstract

American silk moth, Antheraea polyphemus Cramer 1775 (Lepidoptera: Saturniidae), native to North America, has potential significance in sericulture for food consumption and silk production. To date, the phylogenetic relationship and divergence time of A. polyphemus with its Asian relatives remain unknown. To end these issues, two mitochondrial genomes (mitogenomes) of A. polyphemus from the USA and Canada respectively were determined. The mitogenomes of A. polyphemus from the USA and Canada were 15,346 and 15,345 bp in size, respectively, with only two transitions and five indels. The two mitogenomes both encoded typical mitochondrial 37 genes. No tandem repeat elements were identified in the A+T-rich region of A. polyphemus. The mitogenome-based phylogenetic analyses supported the placement of A. polyphemus within the genus Antheraea, and revealed the presence of two clades for eight Antheraea species used: one included A. polyphemus, A. assamensis Helfer, A. formosana Sonan and the other contained A. mylitta Drury, A. frithi Bouvier, A. yamamai Guérin-Méneville, A. proylei Jolly, and A. pernyi Guérin-Méneville. Mitogenome-based divergence time estimation further suggested that the dispersal of A. polyphemus from Asia into North America might have occurred during the Miocene Epoch (18.18 million years ago) across the Berling land bridge. This study reports the mitogenome of A. polyphemus that provides new insights into the phylogenetic relationship among Antheraea species and the origin of A. polyphemus.