Antibacterial activity of diazepam against planktonic and biofilm strains of methicillin-resistant Staphylococcus aureus.

IF 2 3区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biofouling Pub Date : 2025-09-01 Epub Date: 2025-08-01 DOI:10.1080/08927014.2025.2540534

Thaís Lima Ferreira, Lívia Gurgel do Amaral Valente Sá, Vitória Pessoa de Farias Cabral, Daniel Sampaio Rodrigues, Lara Elloyse Almeida Moreira, Beatriz Oliveira de Souza, Bruno Coêlho Cavalcanti, Hemerson Iury Ferreira Magalhães, Iri Sandro Pampolha Lima, Amanda Cavalcante Leitão, Manoel Odorico de Moraes, João Batista de Andrade Neto, Hélio Vitoriano Nobre Júnior, Cecília Rocha da Silva

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Abstract

The aim was to evaluate the antibacterial activity of diazepam against methicillin-susceptible (MSSA) and methicillin-resistant (MRSA) strains of Staphylococcus aureus and its possible mechanism of action. The broth microdilution assay was used to determine the minimum inhibitory concentration (MIC) of diazepam. A checkerboard assay was used to evaluate the interaction of diazepam with different antibiotics. Colorimetric assays with MTT were used to evaluate the effect of diazepam against the biofilms by MSSA and MRSA. Flow cytometry and fluorescence microscopy were used to evaluate the possible mechanism of action of diazepam against MRSA. Diazepam had a MIC of 256 µg/mL. It only had indifferent interactions with the analyzed antibiotics. Diazepam significantly reduced the viability of MSSA and MRSA biofilms. Diazepam caused fragmentation of bacterial DNA and carbonylation of proteins, resulting in reduced cell viability. Therefore, diazepam has in vitro antimicrobial activity against planktonic and biofilm strains of MRSA and MSSA.

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地西泮对耐甲氧西林金黄色葡萄球菌浮游菌和生物膜菌的抑菌活性。

目的评价地西泮对金黄色葡萄球菌甲氧西林敏感（MSSA）和耐甲氧西林（MRSA）菌株的抑菌活性及其可能的作用机制。采用微量肉汤稀释法测定地西泮的最低抑菌浓度（MIC）。采用棋盘法评价地西泮与不同抗生素的相互作用。采用MTT比色法评价地西泮对msa和MRSA生物膜的影响。采用流式细胞术和荧光显微镜观察地西泮对MRSA的可能作用机制。地西泮的MIC为256µg/mL。它与所分析的抗生素仅具有无关的相互作用。地西泮显著降低了MSSA和MRSA生物膜的活力。安定引起细菌DNA的断裂和蛋白质的羰基化，导致细胞活力降低。因此，地西泮对MRSA和MSSA的浮游和生物膜菌株具有体外抗菌活性。

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来源期刊

Biofouling 生物-海洋与淡水生物学

CiteScore

5.00

自引率

7.40%

发文量

审稿时长

1.7 months

期刊介绍： Biofouling is an international, peer-reviewed, multi-discliplinary journal which publishes original articles and mini-reviews and provides a forum for publication of pure and applied work on protein, microbial, fungal, plant and animal fouling and its control, as well as studies of all kinds on biofilms and bioadhesion. Papers may be based on studies relating to characterisation, attachment, growth and control on any natural (living) or man-made surface in the freshwater, marine or aerial environments, including fouling, biofilms and bioadhesion in the medical, dental, and industrial context. Specific areas of interest include antifouling technologies and coatings including transmission of invasive species, antimicrobial agents, biological interfaces, biomaterials, microbiologically influenced corrosion, membrane biofouling, food industry biofilms, biofilm based diseases and indwelling biomedical devices as substrata for fouling and biofilm growth, including papers based on clinically-relevant work using models that mimic the realistic environment in which they are intended to be used.