Leptospiral shedding and seropositivity in asymptomatic stray dogs in Bosnia and Herzegovina.

IF 2.5 3区医学 Q2 PARASITOLOGY

Acta tropica Pub Date : 2025-09-01 Epub Date: 2025-07-29 DOI:10.1016/j.actatropica.2025.107759

Jelena Marić, Ivona Subić, Marija Stojiljković, Branislav Stanković, Sonja Obrenović

{"title":"Leptospiral shedding and seropositivity in asymptomatic stray dogs in Bosnia and Herzegovina.","authors":"Jelena Marić, Ivona Subić, Marija Stojiljković, Branislav Stanković, Sonja Obrenović","doi":"10.1016/j.actatropica.2025.107759","DOIUrl":null,"url":null,"abstract":"<p><p>Leptospirosis is a (re) emerging zoonosis that occurs worldwide. Dogs could develop infections that range from asymptomatic to severe, and shed leptospires with their urin. The aim of this study was to compare the results of real-time polymerase chain reaction (qPCR) by examining urine samples, and serological examination of the blood of stray dogs by the microscopic agglutination test (MAT) on ten serovars of Leptospira spp. A total of 100 blood samples and 50 urine samples of clinically healthy stray dogs were collected from June to November 2024 in the Banja Luka region of Bosnia and Herzegovina. Positive MAT titres (≥ 100) were detected in 15,0 % (15/100) of samples. Dogs were most commonly reactive to Australis (60,0 %) followed by Pomona (26,67 %) Bratislava (20.0 %), Canicola (13,33 %) and 6,67 % for Icterohaemorrhagiae, Grippotyphosa and Sejroe serovars. A positive result of the qPCR test was found in 11 (22.0 %) dogs, with only one of these dogs was positive in the MAT test. It has been found that MAT is not a good method for identifying dogs that shed leptospires in their urine. The study indicates a significant prevalence of leptospirosis in clinically healthy dogs. Therefore, it is important to implement appropriate control measures to prevent further spread of the disease in both human and animal populations.</p>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":" ","pages":"107759"},"PeriodicalIF":2.5000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta tropica","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.actatropica.2025.107759","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/7/29 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"PARASITOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Leptospirosis is a (re) emerging zoonosis that occurs worldwide. Dogs could develop infections that range from asymptomatic to severe, and shed leptospires with their urin. The aim of this study was to compare the results of real-time polymerase chain reaction (qPCR) by examining urine samples, and serological examination of the blood of stray dogs by the microscopic agglutination test (MAT) on ten serovars of Leptospira spp. A total of 100 blood samples and 50 urine samples of clinically healthy stray dogs were collected from June to November 2024 in the Banja Luka region of Bosnia and Herzegovina. Positive MAT titres (≥ 100) were detected in 15,0 % (15/100) of samples. Dogs were most commonly reactive to Australis (60,0 %) followed by Pomona (26,67 %) Bratislava (20.0 %), Canicola (13,33 %) and 6,67 % for Icterohaemorrhagiae, Grippotyphosa and Sejroe serovars. A positive result of the qPCR test was found in 11 (22.0 %) dogs, with only one of these dogs was positive in the MAT test. It has been found that MAT is not a good method for identifying dogs that shed leptospires in their urine. The study indicates a significant prevalence of leptospirosis in clinically healthy dogs. Therefore, it is important to implement appropriate control measures to prevent further spread of the disease in both human and animal populations.

查看原文本刊更多论文

波斯尼亚和黑塞哥维那无症状流浪狗的钩端螺旋体脱落和血清阳性。

钩端螺旋体病是一种（重新）出现的人畜共患病，发生在世界各地。狗可能会出现从无症状到严重的感染，并随着尿液排出钩体。本研究于2024年6月至11月在波黑巴尼亚卢卡地区采集临床健康流浪狗血样100份，尿样实时聚合酶链反应（qPCR）检测结果与显微镜凝集试验（MAT）对10种钩端螺旋体血清学检测结果进行比较。15.0%（15/100）的样品检测到MAT阳性滴度（≥100）。犬类最常见的反应是Australis(60.0%)，其次是Pomona（26.67%）、Bratislava（20.0%）、Canicola（13.33%）和ico出血热、带状伤寒和Sejroe血清型（6.67%）。11只（22.0%）犬qPCR阳性，其中只有1只犬MAT阳性。已经发现MAT并不是鉴别狗的尿液中是否含有钩体的好方法。该研究表明钩端螺旋体病在临床健康犬中显著流行。因此，重要的是实施适当的控制措施，以防止疾病在人类和动物群体中进一步传播。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Acta tropica 医学-寄生虫学

CiteScore

5.40

自引率

11.10%

发文量

383

审稿时长

37 days

期刊介绍： Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.