Unveiling the Genetic Diversity and Antimicrobial Resistance Profiles of Salmonella Population From 2016 to 2020 in Thai Canal Water

IF 2.7 4区生物学 Q2 ENVIRONMENTAL SCIENCES

Environmental Microbiology Reports Pub Date : 2025-08-01 DOI:10.1111/1758-2229.70160

Jirachaya Toyting-Hiraishi, Toyotaka Sato, Neunghatai Supha, Yuwanda Thongpanich, Motohiro Horiuchi, Jeewan Thapa, Chie Nakajima, Yasuhiko Suzuki, Fuangfa Utrarachkij

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Abstract

Salmonella is one of the important pathogens causing acute gastroenteritis, and antimicrobial-resistant Salmonella raises a critical public health concern. Canals in Bangkok, Thailand, play a vital role as sources of agricultural and daily water usage. By employing whole genome sequencing to analyse 351 Salmonella genomes isolated between 2016 and 2020, we expanded the understanding of the characteristics and antimicrobial resistance properties of Salmonella enterica found in Bangkok canals, an underrepresented biome in research. Salmonella Agona was the dominant serotype, while S. Typhimurium and its monophasic variant were periodically found. Seven new sequence types (STs) were identified, including STs 11,346, 11,347, 11,348, 11,349, 11,350, 11,351, and 11,352. Seven chromosomal-mediated gene mutations and 50 antimicrobial resistance genes were detected. The three most common resistance genes were tet(A), bla_TEM-1B, and qnrS1. The tet(X4) was first identified in the Salmonella population in Thailand, and mcr-3.1 was also detected. In total, 39.0% of the strains were potentially multidrug-resistant. The strains carried double amino acid substitutions in GyrA and ParC, and a strain with GyrA substitutions and qnrS1 exhibited the strongest resistance to nalidixic acid and ciprofloxacin. Most of the ceftazidime, ceftriaxone, and cefotaxime-resistant strains (66.7%) harboured bla_CTX-M-55. Col(pHAD28) was the predominant plasmid replicon type. Phylogenetic analysis of Salmonella STs 34 and 213 from canal water and the strains from databases showed the possibility of circulation of STs 34 and 213 between canal water and humans in Thailand and worldwide. These findings shed light on the circulation of antimicrobial-resistant pathogens in the environmental water and advocate for incorporating environmental sampling into comprehensive AMR surveillance programmes within a One Health framework.

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2016 - 2020年泰国运河水域沙门氏菌种群遗传多样性及耐药性分析

沙门氏菌是引起急性胃肠炎的重要病原体之一，耐药沙门氏菌引起了严重的公共卫生问题。泰国曼谷的运河作为农业和日常用水的来源发挥着至关重要的作用。通过对2016年至2020年间分离的351个沙门氏菌基因组进行全基因组测序分析，我们扩大了对曼谷运河中发现的肠沙门氏菌的特征和耐药特性的了解，这是一个研究中代表性不足的生物群系。沙门菌为主要血清型，鼠伤寒沙门菌及其单相变体周期性出现。共鉴定出7个新的序列类型（STs），分别为11346、11347、11348、11349、11350、11351和11352。检测到7个染色体介导的基因突变和50个耐药基因。三种最常见的耐药基因为tet(A)、blaem - 1b和qnrS1。tet（X4）首次在泰国沙门氏菌中发现，mcr-3.1也被检测到。总共有39.0%的菌株具有潜在的多重耐药能力。该菌株在GyrA和ParC中携带双氨基酸替换，其中GyrA和qnrS1替换的菌株对萘啶酸和环丙沙星的抗性最强。头孢他啶、头孢曲松和头孢噻肟耐药菌株中大部分（66.7%）含有blaCTX-M-55。Col（pHAD28）是主要的质粒复制子类型。对运河水中沙门氏菌STs 34和213的系统发育分析和数据库菌株的系统发育分析表明，在泰国和世界范围内，运河水和人之间可能存在STs 34和213的传播。这些发现揭示了环境水中耐抗生素病原体的循环，并倡导将环境抽样纳入“同一个健康”框架内的综合抗菌素耐药性监测规划。

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来源期刊

Environmental Microbiology Reports ENVIRONMENTAL SCIENCES-MICROBIOLOGY

CiteScore

6.00

自引率

3.00%

发文量

审稿时长

3.0 months

期刊介绍： The journal is identical in scope to Environmental Microbiology, shares the same editorial team and submission site, and will apply the same high level acceptance criteria. The two journals will be mutually supportive and evolve side-by-side. Environmental Microbiology Reports provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens.