Parity Influences the Immune Profile of Human Milk via Increased T Lymphocyte Content.

IF 2.1 4区医学 Q1 PEDIATRICS

Acta Paediatrica Pub Date : 2025-07-30 DOI:10.1111/apa.70259

Agata Tomaszewska, Klaudia Porębska, Alicja Jeleniewska, Katarzyna Królikowska, Agnieszka Lipińska-Opałka, Agnieszka Rustecka, Robert Zdanowski, Joanna Kalicka, Agata Będzichowska, Jacek Z Kubiak, Bolesław Kalicki

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引用次数: 0

Abstract

Aim: Breast milk contains immune cells, but the factors influencing their composition are not fully understood. The role of maternal reproductive history in shaping this composition has largely been overlooked. The aim of this study was to evaluate the content of leukocytes, stem cells, and lactocytes in the milk of primiparous mothers compared to multiparous mothers.

Methods: Forty-nine breastfeeding mothers were enrolled in the study: primiparous and multiparous women. Human milk was collected from each participant to assess the content ofthe leukocyte (CD45+), including B lymphocytes (CD19+), NK lymphocytes (CD 16/56+), and T lymphocytes (CD3+), with their subpopulations (CD4+ helper lymphocytes and CD8+ cytotoxic lymphocytes). The percentage of haematopoietic stem cells (CD45-CD105+34+326-), mesenchymal stem cells (CD45-CD105+73+44+) and lactocytes (CD45-CD105+326+) was also determined.

Results: The percentage of lactocytes with the CD326+73- phenotype was significantly higher in multiparous women (95% vs. 89%; p = 0.016), while the percentage of lactocytes with the 326+73+ phenotype was significantly higher in primiparous women (3% vs. 0.7%; p = 0.035). A statistically significantly lower percentage of T helper and T cytotoxic lymphocytes was observed in the first-time mothers (20% vs. 30% respectively; p = 0.049 and 14% vs. 24% respectively; p = 0.006).

Conclusions: Parity was associated with differences in breast milk composition.

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胎次通过增加T淋巴细胞含量影响母乳免疫谱。

目的：母乳中含有免疫细胞，但影响其组成的因素尚不完全清楚。产妇生育史在形成这一构成方面的作用在很大程度上被忽视了。本研究的目的是评估初产母亲与多产母亲乳汁中白细胞、干细胞和乳细胞的含量。方法：49名母乳喂养的母亲参加了这项研究：初产和多产妇女。从每个参与者身上收集母乳，评估白细胞（CD45+）的含量，包括B淋巴细胞（CD19+）、NK淋巴细胞（cd16 /56+）和T淋巴细胞（CD3+）及其亚群（CD4+辅助淋巴细胞和CD8+细胞毒性淋巴细胞）。还测定了造血干细胞（CD45-CD105+34+326-）、间充质干细胞（CD45-CD105+73+44+）和乳细胞（CD45-CD105+326+）的百分比。结果：具有CD326+73-表型的乳细胞百分比在多产妇女中显著更高(95% vs. 89%；P = 0.016)，而326+73+表型的乳细胞百分比在初产妇女中显著更高(3%比0.7%；p = 0.035)。第一次分娩的母亲T辅助淋巴细胞和T细胞毒性淋巴细胞的百分比显著低于第一次分娩的母亲(分别为20%和30%；P = 0.049, 14% vs. 24%；p = 0.006)。结论：胎次与母乳成分的差异有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Paediatrica 医学-小儿科

CiteScore

6.50

自引率

5.30%

发文量

384

审稿时长

2-4 weeks

期刊介绍： Acta Paediatrica is a peer-reviewed monthly journal at the forefront of international pediatric research. It covers both clinical and experimental research in all areas of pediatrics including: neonatal medicine developmental medicine adolescent medicine child health and environment psychosomatic pediatrics child health in developing countries