Haein Oh, Na Yeong Kong, Sung-Won Jung, Hee-Cheol Kim, Shin Kim, Junho Kang, Hojun Lee
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引用次数: 0
Abstract
Objective: Based on the neuroimmunological hypothesis of major depressive disorder (MDD), we analyzed the existing research to identify cytokine-related genes associated with MDD. Furthermore, we examined the cytokine alterations in patients with MDD as potential biomarkers for diagnosis and monitoring.
Methods: Differentially expressed genes (DEGs) related to MDD were identified using the GEO2R tool on public datasets, followed by functional enrichment analyses with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathways. Protein-protein interaction (PPI) networks were constructed using Cytoscape to identify hub genes. Finally, blood samples from 20 patients with MDD and 10 healthy controls were analyzed using the Olink® Target 96 Inflammation panel with proximity extension assay (PEA) technology to identify potential protein biomarkers.
Results: Two GEO datasets related to MDD were analyzed to identify 66 common DEGs. Following the PPI analysis, 46 genes were identified. Functional enrichment analysis revealed that these genes were closely related to immune-related pathways. Subsequent blood sample analysis of patients with MDD and healthy controls confirmed that 18 cytokines related to 46 DEGs were significantly upregulated. Among the identified cytokines, oncostatin M (OSM) showed the highest receiver operating characteristic (ROC) performance (area under the curve [AUC]=0.96), followed by hepatocyte growth factor (HGF) (AUC=0.95), cluster of differentiation 6 (CD6) (AUC=0.90), and tumor necrosis factor superfamily 14 (TNFSF14) (AUC=0.90).
Conclusion: Our study confirms that neuroinflammation is an important pathophysiological aspect of MDD and that several related cytokines, such as OSM, HGF, CD6, and TNFSF14, may be potential biomarkers of MDD.
期刊介绍:
The Psychiatry Investigation is published on the 25th day of every month in English by the Korean Neuropsychiatric Association (KNPA). The Journal covers the whole range of psychiatry and neuroscience. Both basic and clinical contributions are encouraged from all disciplines and research areas relevant to the pathophysiology and management of neuropsychiatric disorders and symptoms, as well as researches related to cross cultural psychiatry and ethnic issues in psychiatry. The Journal publishes editorials, review articles, original articles, brief reports, viewpoints and correspondences. All research articles are peer reviewed. Contributions are accepted for publication on the condition that their substance has not been published or submitted for publication elsewhere. Authors submitting papers to the Journal (serially or otherwise) with a common theme or using data derived from the same sample (or a subset thereof) must send details of all relevant previous publications and simultaneous submissions. The Journal is not responsible for statements made by contributors. Material in the Journal does not necessarily reflect the views of the Editor or of the KNPA. Manuscripts accepted for publication are copy-edited to improve readability and to ensure conformity with house style.