The effect of bioactive compounds extracted from natural rubber serum on wound healing: An in vitro study.

IF 1.3 4区医学 Q4 ENGINEERING, BIOMEDICAL

International Journal of Artificial Organs Pub Date : 2025-07-30 DOI:10.1177/03913988251360554

Prisana Pripatnanont, Kawintip Prasongyuenyong, Jutakan Thonglam, Kanokporn Santavalimp, Thanawat Pitakpornpreecha, Aratee Aroonkesorn

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引用次数: 0

Abstract

Background: Wound healing is a complex process that involves a sequential interplay of inflammation, cell proliferation, and migration. Natural rubber extracts have been explored in cell migration and proliferation, playing a pivotal role in tissue regeneration.

Objectives: This study identified and fractionated bioactive elements in natural rubber serum and investigated their effect on wound healing capacity.

Methods: The fractions were obtained through anion exchange chromatography, acetone precipitation, and ammonium sulfate precipitation. Wound healing effects were studied in vitro through fibroblast cell proliferation, scratch assay, and fibroblast cytotoxicity by live-dead cell staining.

Results: Specific fractions R10k0.2, R10k0.4, R1k1, R1k0.6, and R1k0.8 were obtained through anion chromatography. Only the R1k-unbound, R1k0.6, and R1k0.8 promoted fibroblast proliferation, and those fractions were further extracted by acetone precipitation and ammonium sulfate. These fractions expedited wound gap closure in migration assays. Sugar compounds and carbohydrates were primarily identified in the fractions from anion exchange chromatography, while protein constituents were found from ammonium sulfate precipitation.

Conclusions: The bioactive fractions from natural rubber serum primarily consisted of sugar compounds with trace amounts of proteins. The carbohydrates extracted enhanced fibroblast proliferation and migration with no toxicity to the cells. These compounds possessed potential applications in wound healing and tissue regeneration.

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天然橡胶血清中提取的生物活性化合物对伤口愈合的影响：体外研究。

背景：伤口愈合是一个复杂的过程，涉及炎症、细胞增殖和迁移的连续相互作用。天然橡胶提取物在细胞迁移和增殖中被发现，在组织再生中起着关键作用。目的：对天然橡胶血清中的生物活性元素进行鉴定和分离，探讨其对伤口愈合能力的影响。方法：采用阴离子交换色谱法、丙酮沉淀法、硫酸铵沉淀法提取。通过成纤维细胞增殖、划痕实验和活细胞染色研究成纤维细胞的体外愈合作用。结果：通过阴离子色谱法获得了R10k0.2、R10k0.4、R1k1、R1k0.6、R1k0.8等特异组分。只有r1k -未结合、R1k0.6和R1k0.8对成纤维细胞增殖有促进作用，用丙酮沉淀和硫酸铵进一步提取。这些组分加速了迁移试验中伤口间隙的闭合。糖化合物和碳水化合物主要从阴离子交换色谱中分离出来，而蛋白质成分主要从硫酸铵沉淀中分离出来。结论：天然橡胶血清的生物活性组分主要由糖化合物和微量蛋白质组成。提取的碳水化合物增强了成纤维细胞的增殖和迁移，对细胞没有毒性。这些化合物在伤口愈合和组织再生方面具有潜在的应用前景。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Artificial Organs 医学-工程：生物医学

CiteScore

3.40

自引率

5.90%

发文量

审稿时长

3 months

期刊介绍： The International Journal of Artificial Organs (IJAO) publishes peer-reviewed research and clinical, experimental and theoretical, contributions to the field of artificial, bioartificial and tissue-engineered organs. The mission of the IJAO is to foster the development and optimization of artificial, bioartificial and tissue-engineered organs, for implantation or use in procedures, to treat functional deficits of all human tissues and organs.