Effect of bisphenol-A and bisphenol-S on functional parameters of human leukocytes.

IF 3 4区医学 Q3 IMMUNOLOGY

Immunopharmacology and Immunotoxicology Pub Date : 2025-10-01 Epub Date: 2025-08-12 DOI:10.1080/08923973.2025.2542133

Manuel Iván Girón-Pérez, Guadalupe Herminia Ventura-Ramón, Carlos Eduardo Covantes-Rosales, Alma Betsaida Benitez-Trinidad, Francisco Fabian Razura-Carmona, Leticia Gabriela Marmolejo-Murillo, Carlos Efren Pérez-Arenivas, Jorge Morales-Montor, Karina Janice Guadalupe Díaz-Resendiz

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引用次数: 0

Abstract

Background: Bisphenol A (BPA) and bisphenol S (BPS) are commonly used in the food industry to manufacture epoxy resins in food packaging. Both compounds are characterized as potent carcinogens and xenoestrogens. However, little is known about their effects on the immune response.

Objective: This study evaluated the immunotoxic effects of BPA and BPS (0.1 ng/mL) on human peripheral blood leukocytes.

Methods: Leukocytes isolated from human peripheral blood were exposed in vitro to 0.1 ng/mL (0.4 nM) of BPA and 0.1 ng/mL (0.4 nM) of BPS for 0.5, 4, and 24 hours. Apoptosis, mitochondrial membrane potential (ΔΨm), senescence, reactive oxygen species (ROS), and intracellular Ca²⁺ flux were subsequently assessed through flow cytometry.

Results: Data suggest that in vitro exposure to BPA or BPS does not cause leukocyte death; however, it induces loss of mitochondrial membrane potential (ΔΨm), senescence, as well as ROS production and intracellular Ca²⁺ flux.

Conclusion: These findings demonstrate that even low concentrations (0.1 ng/ml) of BPA or BPS, levels that have been reported in human blood, can cause alterations in leukocyte function.

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双酚a和双酚s对人白细胞功能参数的影响。

双酚A （BPA）和双酚S （BPS）在食品工业中常用来制造食品包装中的环氧树脂。这两种化合物都被认为是强致癌物和雌激素。然而，人们对它们对免疫反应的影响知之甚少。本研究评价了BPA和BPS （0.1 ng/mL）对人外周血白细胞的免疫毒性作用。数据表明，体外暴露于BPA或BPS不会导致白细胞死亡；然而，它会导致线粒体膜电位的丧失（ΔΨm）、衰老，以及活性氧产生和细胞内Ca2+通量的增加。这些发现表明，即使在人体血液中检测到低浓度（0.1 ng/mL）的BPA或BPS也会导致白细胞功能的改变。这些改变可能通过导致自身耐受性、自身免疫力的丧失和炎症性疾病（如糖尿病、心血管疾病甚至癌症）的发展来潜在地调节免疫反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Immunopharmacology and Immunotoxicology 医学-毒理学

CiteScore

5.40

自引率

0.00%

发文量

133

审稿时长

4-8 weeks

期刊介绍： The journal Immunopharmacology and Immunotoxicology is devoted to pre-clinical and clinical drug discovery and development targeting the immune system. Research related to the immunoregulatory effects of various compounds, including small-molecule drugs and biologics, on immunocompetent cells and immune responses, as well as the immunotoxicity exerted by xenobiotics and drugs. Only research that describe the mechanisms of specific compounds (not extracts) is of interest to the journal. The journal will prioritise preclinical and clinical studies on immunotherapy of disorders such as chronic inflammation, allergy, autoimmunity, cancer etc. The effects of small-drugs, vaccines and biologics against central immunological targets as well as cell-based therapy, including dendritic cell therapy, T cell adoptive transfer and stem cell therapy, are topics of particular interest. Publications pointing towards potential new drug targets within the immune system or novel technology for immunopharmacological drug development are also welcome. With an immunoscience focus on drug development, immunotherapy and toxicology, the journal will cover areas such as infection, allergy, inflammation, tumor immunology, degenerative disorders, immunodeficiencies, neurology, atherosclerosis and more. Immunopharmacology and Immunotoxicology will accept original manuscripts, brief communications, commentaries, mini-reviews, reviews, clinical trials and clinical cases, on the condition that the results reported are based on original, clinical, or basic research that has not been published elsewhere in any journal in any language (except in abstract form relating to paper communicated to scientific meetings and symposiums).