Coenzyme Q10 ameliorates anxiety and depression‑like behavior associated with chronic opioid use and increases GDNF expression in the hippocampus of morphine‑dependent rats.

IF 1.4 4区 医学 Q4 NEUROSCIENCES
Mobina Gheibi, Somayeh Nazari, Fahimeh Mohseni, Pooria Salehi Sangani, Farzaneh Felehkari, Saba Niknamfar, Hamed Ghazvini, Seyedeh Masoumeh Seyedhosseini Tamijani, Hamid Kalalian-Moghaddam, Hamid Jomehpour, Raheleh Rafaiee
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Abstract

Opioid dependence is strongly associated with moderate to severe depression and anxiety. The primary objective of this investigation was to determine whether coenzyme Q10 (CoQ10) has the capacity to increase the level of glial cell line‑derived neurotrophic factor (GDNF), with the aim of ameliorating anxiety‑ and depression‑like behaviors in morphine (MOP)‑dependent rats. In this study, 40 male Wistar rats were randomly divided into five experimental groups: Oil group, MOP+Oil group, MOP+Q10‑100 group, MOP+Q10‑200 group, and MOP+Q10‑400 group. Rats received escalating doses of MOP (25 to 100 mg/kg, s.c.) once daily. After 21 days of drug dependency, CoQ10 was administered orally at doses of 100, 200, and 400 mg/kg once daily for four weeks. Behavioral assessments were conducted using the open field test, elevated plus maze, and forced swim test. GDNF expression in the hippocampus was evaluated using immunohistochemistry. Four weeks of CoQ10 treatment significantly improved anxiety‑ and depression‑like behaviors induced by MOP administration. Furthermore, CoQ10 significantly increased GDNF expression in the hippocampus. Oral administration of CoQ10 at doses of 100, 200, and 400 mg/kg over four weeks significantly reduced depressive‑ and anxiety‑related behaviors associated with prolonged MOP exposure. These behavioral improvements were accompanied by increased hippocampal GDNF expression.

辅酶Q10改善与慢性阿片类药物使用相关的焦虑和抑郁样行为,并增加吗啡依赖大鼠海马中的GDNF表达。
阿片类药物依赖与中度至重度抑郁和焦虑密切相关。本研究的主要目的是确定辅酶Q10 (CoQ10)是否具有提高神经胶质细胞系来源的神经营养因子(GDNF)水平的能力,以改善吗啡依赖大鼠的焦虑和抑郁样行为。本研究将40只雄性Wistar大鼠随机分为5个实验组:Oil组、MOP+Oil组、MOP+Q10‑100组、MOP+Q10‑200组、MOP+Q10‑400组。给药剂量逐渐递增(25 ~ 100 mg/kg, s.c),每日1次。药物依赖21天后,辅酶q10以100,200和400mg /kg的剂量口服,每天一次,持续四周。行为学评估采用开阔场试验、高架迷宫试验和强迫游泳试验。免疫组化法检测海马组织中GDNF的表达。4周的CoQ10治疗显著改善了MOP诱导的焦虑和抑郁样行为。此外,CoQ10显著增加海马中GDNF的表达。口服辅酶q10 100、200和400 mg/kg,持续4周,显著降低与长时间接触MOP相关的抑郁和焦虑相关行为。这些行为改善伴随着海马GDNF表达的增加。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
2.20
自引率
7.10%
发文量
40
审稿时长
>12 weeks
期刊介绍: Acta Neurobiologiae Experimentalis (ISSN: 0065-1400 (print), eISSN: 1689-0035) covers all aspects of neuroscience, from molecular and cellular neurobiology of the nervous system, through cellular and systems electrophysiology, brain imaging, functional and comparative neuroanatomy, development and evolution of the nervous system, behavior and neuropsychology to brain aging and pathology, including neuroinformatics and modeling.
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