Genomic analysis and molecular profiling of dengue viruses in Sabah, Malaysia during 2019-2020

IF 1.7 Q4 INFECTIOUS DISEASES

IJID regions Pub Date : 2025-07-18 DOI:10.1016/j.ijregi.2025.100712

Mya Myat Ngwe Tun , Jecelyn Leaslie John , Takeshi Nabeshima , Abdul Marsudi Manah , Yuki Takamatsu , Takeshi Urano , Kouichi Morita , Kamruddin Ahmed

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Abstract

Objectives

Limited information is available on the distribution of dengue virus (DENV) serotypes and genotypes in Borneo, particularly, in Sabah, because most studies have focused on Peninsular Malaysia. This study aimed to investigate the serologic and molecular epidemiologic characteristics of DENV in patients from Sabah.

Methods

Serum samples were collected from febrile patients in Kota Kinabalu and Lahad Datu between 2019 and 2020 at the Kota Kinabalu Public Health Laboratory. We performed virus isolation, serological testing, viremia quantification, and complete genome sequencing.

Results

Of 188 serum samples, 89 tested positive for DENV by quantitative reverse transcription-polymerase chain reaction: 20 DENV-1, 46 DENV-2, 20 DENV-3, and 1 DENV-4. A total of 38 viruses were successfully isolated. The isolates belonged to DENV-1 (genotypes I and IV), DENV-2 (cosmopolitan genotype) and DENV-3 (genotypes I and III). The phylogenetic analysis revealed close relationships with strains from Malaysia, Indonesia, and the Philippines. DENV-2 was the predominant serotype, whereas the highest viremia levels were observed in patients infected with DENV-3.

Conclusions

To the best of our knowledge, this study provides the first detailed phylogenetic and complete genome analysis of DENV in Sabah. Continued molecular surveillance is essential to enhance our understanding of dengue transmission and support effective control strategies.

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2019-2020年马来西亚沙巴登革热病毒的基因组分析和分子图谱

目的关于登革热病毒（DENV）血清型和基因型在婆罗洲分布的信息有限，特别是在沙巴，因为大多数研究都集中在马来西亚半岛。本研究旨在调查沙巴州DENV患者的血清学和分子流行病学特征。方法在哥打京那巴鲁公共卫生实验室采集2019 - 2020年哥打京那巴鲁和拉哈德拿都发热患者的血清样本。我们进行了病毒分离、血清学检测、病毒血症定量和全基因组测序。结果188份血清样本中，DENV定量逆转录聚合酶链反应阳性89份，其中DENV-1 20份，DENV-2 46份，DENV-3 20份，DENV-4 1份。总共成功分离出38种病毒。分离株分别为DENV-1（基因型I和IV）、DENV-2（世界性基因型）和DENV-3（基因型I和III）。系统发育分析显示与来自马来西亚、印度尼西亚和菲律宾的菌株关系密切。DENV-2为主要血清型，而DENV-3感染患者的病毒血症水平最高。结论据我们所知，本研究首次提供了沙巴州DENV的详细系统发育和全基因组分析。持续的分子监测对于加强我们对登革热传播的了解和支持有效的控制战略至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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