HPLC-UV guided isolation of anti-inflammatory polyacetylene glucosides from Carthamus tinctorius L.

IF 3.4 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Phytochemistry Pub Date : 2025-07-29 DOI:10.1016/j.phytochem.2025.114629

Yong-Xiang Wang , Jia-Xu Bao , Ran Wang , Xian Zhang , Yun-Fang Zhao , Peng-Fei Tu , Hui-Xia Huo , Jun Li

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Abstract

HPLC-UV guided fractionation led to the isolation of nineteen previously undescribed polyacetylene glucosides (1–19) from the florets of Carthamus tinctorius L. Their structures, including the absolute configurations, were established by analysis of UV, IR, HRESIMS, 1D and 2D NMR data, together with enzymatic hydrolysis and ECD calculations. Compounds 1 and 2 were polyacetylene glucosides bearing an unusual furan ring. Compounds 3–6 were rare polyacetylene glucosides with an α-l-arabinofuranosyl moiety, while compound 3 comprised a 1-O-α-l-arabinofuranosyl-(1 → 3)-β-d-glucopyranosyl disaccharide unit. Compounds 2, 4, 5, 8, 11–13, 16, and 18 showed inhibition of nitric oxide secretion in lipopolysaccharide-stimulated RAW264.7 cells with IC₅₀ values ranging from 5.0 to 40.0 μM.

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HPLC-UV引导下红花抗炎聚乙炔苷的分离。

通过HPLC-UV引导分离，从红花（Carthamus tinctorius L.）小花中分离到19个先前未被描述的聚乙炔糖苷（1-19），通过UV、IR、hremsims、1D和2D NMR数据分析，并结合酶解和ECD计算，确定了它们的结构，包括绝对构型。化合物1和2是带有不寻常的呋喃环的聚乙炔糖苷。化合物3 - 6是含有α-l-阿拉伯糖醛基片段的罕见聚乙炔糖苷，而化合物3由1- o -α-l-阿拉伯糖醛基-(1→3)-β-d-葡萄糖醛基双糖单元组成。化合物2、4、5、8、11-13、16和18对脂多糖刺激RAW264.7细胞的一氧化氮分泌有抑制作用，IC50值在5.0 ~ 40.0 μM之间。

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来源期刊

Phytochemistry 生物-植物科学

CiteScore

6.40

自引率

7.90%

发文量

443

审稿时长

39 days

期刊介绍： Phytochemistry is a leading international journal publishing studies of plant chemistry, biochemistry, molecular biology and genetics, structure and bioactivities of phytochemicals, including ''-omics'' and bioinformatics/computational biology approaches. Phytochemistry is a primary source for papers dealing with phytochemicals, especially reports concerning their biosynthesis, regulation, and biological properties both in planta and as bioactive principles. Articles are published online as soon as possible as Articles-in-Press and in 12 volumes per year. Occasional topic-focussed special issues are published composed of papers from invited authors.