Mechanisms and Strategies for Engineering Oxidative Stress Resistance in Saccharomyces cerevisiae

Abstract

Oxidative stress, driven by the accumulation of reactive oxygen species (ROS), poses a significant challenge to the productivity and robustness of Saccharomyces cerevisiae in industrial applications. This review provides an overview of oxidative stress mechanisms, focusing on transcription factors (Yap1p, Skn7p, Msn2/4p) and their regulation through different stress signaling pathways such as HOG, CWI, TOR, and cAMP/PKA. Advanced strategies for enhancing oxidative stress resistance are discussed, including antioxidant enzyme overexpression, redox cofactor optimization, transcription factor modulation, and promoter engineering. Emerging tools like omics-guided gene discovery, biosensor-based feedback regulation, and machine learning-driven optimization are highlighted as promising approaches for constructing robust yeast cell factories. These insights pave the way for intelligent strain design to improve industrial performance under oxidative stress conditions.